AI Article Synopsis
- Vaccines can be contaminated by unknown agents, often originating from historical vaccine seeds with unclear backgrounds.
- Researchers created synthetic poliovirus seeds from DNA plasmids that mimic the wild-type strains used in existing vaccines, showing they were just as effective in cell cultures.
- Using these synthetic seeds grown in a controlled, serum-free environment allows for better documentation and control of the starting materials, enhancing vaccine safety by reducing the risk of contamination.
Article Abstract
Safety of vaccines can be compromised by contamination with adventitious agents. One potential source of adventitious agents is a vaccine seed, typically derived from historic clinical isolates with poorly defined origins. Here we generated synthetic poliovirus seeds derived from chemically synthesized DNA plasmids encoding the sequence of wild-type poliovirus strains used in marketed inactivated poliovirus vaccines. The synthetic strains were phenotypically identical to wild-type polioviruses as shown by equivalent infectious titers in culture supernatant and antigenic content, even when infection cultures are scaled up to 10-25L bioreactors. Moreover, the synthetic seeds were genetically stable upon extended passaging on the PER.C6 cell culture platform. Use of synthetic seeds produced on the serum-free PER.C6 cell platform ensures a perfectly documented seed history and maximum control over starting materials. It provides an opportunity to maximize vaccine safety which increases the prospect of a vaccine end product that is free from adventitious agents.
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| http://dx.doi.org/10.1016/j.vaccine.2015.08.081 | DOI Listing |
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