AI Article Synopsis
- Understanding how Arf GAPs interact with specific Arfs is crucial for deciphering their roles in the endocytic system.
- While cell-based methods have helped identify active Arfs and Arf GAPs in this compartment, they have limitations in clarifying their relationships.
- This text outlines a straightforward in vitro assay designed to compare Arfs as substrates, providing additional insights that complement existing cell-based research.
Article Abstract
Defining the interaction of Arf GAPs with specific Arfs is important for understanding their functions in the endocytic system. Cell-based approaches have been valuable for identifying Arfs and Arf GAPs active in the endocytic compartment; however, the cell-based assays have some limitations in establishing relationships among the Arfs and ArfGAPs. Here we describe a simple in vitro assay that will provide a means for comparing Arfs as substrates and serve to complement cell-based studies.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7743909 | PMC |
| http://dx.doi.org/10.1016/bs.mcb.2015.03.021 | DOI Listing |
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