Purpose: The purpose of this study was to evaluate the acid resistance of enamel subsurface lesions treated with casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF).
Methods: Fifty extracted primary molars with a standardized window on enamel were immersed in a demineralizing solution for 72 hours to produce subsurface enamel lesions. They were sectioned in a buccolingual direction; one half of the sample was treated with the remineralizing agent CPP-ACPF and the other half remained untreated (control group). After 10 days, the sample was evaluated quantitatively using energy dispersive X-ray spectroscopy. The treated samples were reimmersed into the demineralizing solution for 72 hours and the mineral content was re-evaluated.
Results: The mean calcium content of the remineralized samples was significantly higher than that of the control group (P<.001) and was higher than the second demineralization values. The mean calcium content of the second demineralization was significantly higher than those of the control group (P<.001).
Conclusion: CPP-ACPF increased the resistance of enamel surfaces to further demineralization.
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