The method for analysis of chlorophyll fluorescence transient using approximation of measured signal by multi-exponential series is described. Visualization of partial sums of this series allows us to find amplitudes and characteristic times of individual phases of fluorescence induction curve. This method gives more rigid criteria of phase identification instead of semi-empirical approach currently used. Applied to Chlamidomonas reinhardtii sulfur deprivation case, it shows efficiency in finding visually undistinguishable phases of fluorescence transient for early detection of stress.
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