The chemical synthesis of the 184-residue ferric heme-binding protein nitrophorin 4 was accomplished by sequential couplings of five unprotected peptide segments using α-ketoacid-hydroxylamine (KAHA) ligation reactions. The fully assembled protein was folded to its native structure and coordinated to the ferric heme b cofactor. The synthetic holoprotein, despite four homoserine residues at the ligation sites, showed identical properties to the wild-type protein in nitric oxide binding and nitrite dismutase reactivity. This work establishes the KAHA ligation as a valuable and viable approach for the chemical synthesis of proteins up to 20 kDa and demonstrates that it is well-suited for the preparation of hydrophobic protein targets.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/anie.201505379 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reaction mechanism with indano[60]fullerene bromide for evaporable fullerene derivatives.
Chem Commun (Camb)
January 2025
Department of Chemical Systems Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Indano[60]fullerene bromide was isolated as a key intermediate in the synthesis of indano[60]fullerene ketone, an evaporable fullerene derivative. Based on the identified reaction mechanism, aqueous hydrobromic acid was used to achieve reproducible and high-yielding reactions. A scalable one-pot reaction from aryl[60]fullerenyl dimers to indano[60]fullerene ketone was also demonstrated.
View Article and Find Full Text PDFSelenium-assisted copper-catalyzed synthesis of phosphoramide from secondary phosphine oxide involving the construction of N-P bonds.
Org Biomol Chem
January 2025
College of Chemical Engineering, Sichuan University of Science & Engineering, Zigong 643000, China.
In this study, phosphoramide compounds were successfully synthesized a series of reaction transformations from P(O)H compounds. The process began with the formation of P-Se-Ar bonds, facilitated by the synergistic effect of phenylboronic acid, selenium, and appropriate ligands in the presence of copper. Following this, nucleophilic substitution reactions with amine compounds were conducted to create P-N bonds.
View Article and Find Full Text PDFPrinting rare-earth-free (REF) magnetic inks: synthesis, formulation, and device applications.
Nanoscale
January 2025
Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.
Additive manufacturing (AM) of magnetic materials has recently attracted increasing interest for various applications but is often limited by the high cost and supply chain risks of rare-earth-element (REE) magnetic precursors. Recent advances in nanomanufacturing have enabled the development of rare-earth-free (REF) magnetic materials, such as spinel ferrites, hexaferrites, MnAl, MnBi, Alnico, FePt, and iron oxides/nitrides, which offer promising alternatives for printing high-performance magnetic devices. This review provides a detailed overview of the latest developments in REF magnetic materials, covering both synthesis strategies of REF magnetic materials/nanomaterials and their integration into AM processes.
View Article and Find Full Text PDFPhotoinduced synthesis of trisubstituted allylic molecules migratory allylation of olefins.
Chem Commun (Camb)
January 2025
Shanghai Frontiers Science Center for Chinese Medicine Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Shanghai 201203, China.
A transition-metal-free method to afford diverse trisubstituted allylic molecules migratory allylation of olefins under light irradiation is described. This system tolerated diverse -tosylhydrazones and olefinic boronic acids. Successful allylation of drugs and natural-product analogues was achieved.
View Article and Find Full Text PDFRecent advances in the reactions of isatin-derived MBH carbonates for the synthesis of spirooxindoles.
Org Biomol Chem
January 2025
Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China.
As one of the main fragments in medical drugs, spirooxindole has received considerable attention from organic and medicinal chemists. In the past few decades, chemists have been searching for more straightforward and efficient methods to produce compounds containing a spirooxindole fragment. In this regard, isatin-derived Morita-Baylis-Hillman (MBH) carbonates have been widely used as versatile building blocks for the synthesis of spirooxindole structures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!