A real-time documentation and mechanistic investigation of quantum dots-induced autophagy in live Caenorhabditis elegans.

Biomaterials

Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China. Electronic address:

Published: December 2015

Autophagy is a highly important intracellular process for the degradation of endogenous or foreign contents in the cytoplasm. Though nanomaterials-induced autophagy has been extensively studied, real-time information about the autophagic process induced by nanomaterials in live organisms remains unknown. Here by using Caenorhabditis elegans as the model organism and fluorescent semiconductor quantum dots (QDs) as a representative nanomaterial, we systematically investigated the phenomenon of QDs-induced autophagy in live organisms. Our results demonstrated that the internalized QDs trigger a complete autophagic process in C. elegans intestinal cells. Further investigations revealed that this QD-induced autophagy in C. elegans is neither a response to released heavy metal ions by the QDs, nor an attempt to engulf exogenous QD materials, but a defensive strategy of the organism to clear and recycle damaged endosomes. Of particular significance, for the first time, we presented real-time tracking of autophagosomes formation in live organisms, providing detailed temporal-spatial information of this process. This study may help us better understand the relationship between nanomaterials and autophagy in vivo, and provide invaluable information for safety evaluation and bio-application of nanomaterials.

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http://dx.doi.org/10.1016/j.biomaterials.2015.08.044DOI Listing

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