Aqueous solutions of tetrabutylphosphonium hydroxide have been evaluated as pretreatment media for rice husks, prior to sulphuric acid hydrolysis or cellulase enzymatic hydrolysis. Varying the water:tetrabutylphosphonium hydroxide ratio varied the rate of delignification, as well as silica, lignin and cellulose solubility. Pre-treatment with 60wt% hydroxide dissolved the rice husk and the regenerated material was thus heavily disrupted. Sulphuric acid hydrolysis of 60wt%-treated samples yielded the highest amount of glucose per gram of rice husk. Solutions with good lignin and silica solubility but only moderate to negligible cellulose solubility (10-40wt% hydroxide) were equally effective as pre-treatment media for both acid and enzymatic hydrolysis. However, pre-treatment with 60wt% hydroxide solutions was incompatible with downstream enzymatic hydrolysis. This was due to significant incorporation of phosphonium species in the regenerated biomass, which significantly inhibited the activity of the cellulase enzymes.
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http://dx.doi.org/10.1016/j.biortech.2015.08.056 | DOI Listing |
J Agric Food Chem
January 2025
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, P. R. China.
In the current work, lychee pulp was subjected to ATCC 14917 fermentation, leading to a substantial increase (2.32-2.67-fold) in water-soluble polysaccharides (WSP).
View Article and Find Full Text PDFWater Res X
May 2025
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Although the treatment of sludge with free nitrous acid can effectively recover short chain fatty acids, the feasibility of sequential nitrite reduction and methane recovery without acidic pH adjustment is still scarcely studied. Therefore, this study aimed to provide insights into the effect of nitrite at different levels on nitrite reduction and methane production. The results showed that the nitrite concentrations of 100, 200, 400 and 800 mg/L were completely reduced in 1, 2, 2 and 4 days, respectively.
View Article and Find Full Text PDFFood Chem X
January 2025
Guiyang University, Guiyang 550005, China.
This study investigates the effect of 100 mg L thymol treatment on the quality of post-harvest peppers stored at 10 °C. The results showed that thymol treatment significantly reduced decay rate, reactive oxygen species (ROS) accumulation, and saturated fatty acid levels in peppers. Moreover, unsaturated fatty acids, non-enzymatic antioxidants, and antioxidant enzyme levels increased after treatment.
View Article and Find Full Text PDFFront Mol Biosci
January 2025
Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Introduction: This study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides.
Methods: The properties of hydrolysates were characterized, including degree of hydrolysis, free amino acids, SDS-PAGE, FTIR, and antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, and FRAP assay.
Results: BLG tryptic hydrolysate produced after 60 min hydrolysis recorded the highest antioxidant activity, and LCMS analysis revealed 162 peptides of molecular masses ranging from 800 to 5671Da, most of them are of hydrophobic nature.
J Agric Food Chem
January 2025
Institute of Quality Standard and Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China.
γ-l-Glutamyl-S-allyl-l-cysteine (GSAC) is renowned for its flavor-modifying effects and beneficial biological activities. However, the level of GSAC decreases significantly during the processing of black garlic, and the pathways and degradation products resulting from this decline remain unclear. To investigate the potential transformation mechanisms of GSAC in black garlic, simulation systems for thermal decomposition, Maillard reactions, and enzymatic hydrolysis were established.
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