AI Article Synopsis
- This study investigates the development of a chitosan hydrogel combined with hydroxypropyl methylcellulose (HPMC) and toluidine blue O (TBO) to enhance its effectiveness as an antimicrobial treatment for skin infections.
- Results show that the hydrogel can effectively kill bacteria like Staphylococcus aureus and Pseudomonas aeruginosa, but higher concentrations of HPMC may limit TBO's ability to penetrate bacterial biofilms.
- The findings suggest that the improved retention of the hydrogel in the biofilm and its application in animal models can significantly boost its bactericidal action when used alongside light irradiation.
Article Abstract
Photodynamic inactivation (PDI) combined with chitosan has been shown as a promising antimicrobial approach. The purpose of this study was to develop a chitosan hydrogel containing hydroxypropyl methylcellulose (HPMC), chitosan and toluidine blue O (TBO) to improve the bactericidal efficacy for topical application in clinics. The PDI efficacy of hydrogel was examined in vitro against the biofilms of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). Confocal scanning laser microscopy (CSLM) was performed to investigate the penetration level of TBO into viable S. aureus biofilms. Incorporation of HMPC could increase the physicochemical properties of chitosan hydrogel including the hardness, viscosity as well as bioadhesion; however, higher HMPC concentration also resulted in reduced antimicrobial effect. CSLM analysis further demonstrated that higher HPMC concentration constrained TBO diffusion into the biofilm. The incubation of biofilm and hydrogel was further performed at an angle of 90 degrees. After light irradiation, compared to the mixture of TBO and chitosan, the hydrogel treated sample showed increased PDI efficacy indicated that incorporation of HPMC did improve antimicrobial effect. Finally, the bactericidal efficacy could be significantly augmented by prolonged retention of hydrogel in the biofilm as well as in the animal model of rat skin burn wounds after light irradiation.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613232 | PMC |
| http://dx.doi.org/10.3390/ijms160920859 | DOI Listing |
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