AI Article Synopsis
- The study sequenced the genome of Pandoraea pnomenusa RB38 using advanced PacBio SMRT sequencing technology.
- It identified a pair of luxI/R homologs (ppnI and ppnR1) and an orphan homolog (ppnR2), with ppnI functioning as an AHL synthase gene distinct from related bacteria.
- High-resolution mass spectrometry confirmed that ppnI directs the production of a specific AHL, marking the first documentation of these luxI/R homologs in the Pandoraea genus.
Article Abstract
In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate luxI/R homologs was identified where the luxI homolog, ppnI, was found adjacent to a luxR homolog, ppnR1. An additional orphan luxR homolog, ppnR2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene that is distinct from those of the nearest phylogenetic neighbor viz. Burkholderia spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that Escherichia coli BL21 harboring ppnI produced a similar AHL profile (N-octanoylhomoserine lactone, C8-HSL) as P. pnomenusa RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in P. pnomenusa RB38. To our knowledge, this is the first documentation of the luxI/R homologs of the genus Pandoraea.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556143 | PMC |
| http://dx.doi.org/10.7717/peerj.1225 | DOI Listing |
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