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Recapitulating Glioma Stem Cell Niches Using 3D Spheroid Models for Glioblastoma Research.
Biosensors (Basel)
November 2024
Department of Pharmacology, Wonkwang University School of Medicine, Iksan 54538, Republic of Korea.
Glioblastoma multiforme (GBM) is among the most aggressive brain cancers, and it contains glioma stem cells (GSCs) that drive tumor initiation, progression, and recurrence. These cells resist conventional therapies, contributing to high recurrence rates in GBM patients. Developing in vitro models that mimic the tumor microenvironment (TME), particularly the GSC niche, is crucial for understanding GBM growth and therapeutic resistance.
View Article and Find Full Text PDFCombined response of polar magnetotaxis to oxygen and pH: Insights from hanging drop assays and microcosm experiments.
Sci Rep
November 2024
Key Laboratory for Humid Subtropical Ecogeographical Processes of the Ministry of Education, Fujian Normal University, Fuzhou, 350117, China.
Article Synopsis
- Magnetotactic bacteria (MTB) utilize both magnetic field alignment and a chemotactic response to navigate to their ideal living depths in environments with varying chemical compositions.
- Current models of magneto-aerotaxis don’t accurately explain why some MTB are found below the oxic-anoxic interface or how different groups coexist at these depths despite their opposing magnetic polarities.
- A proposed new model suggests that MTB's magnetotactic behavior is influenced by specific gradients of oxygen and other substances, allowing them to navigate effectively across these gradients and adapt to varied ecological environments.
Inter-Skeleton Conductive Routes Tuning Multifunctional Conductive Foam for Electromagnetic Interference Shielding, Sensing and Thermal Management.
Nanomicro Lett
October 2024
School of Materials Science and Engineering, Beihang University, Beijing, 100191, People's Republic of China.
Conductive polymer foam (CPF) with excellent compressibility and variable resistance has promising applications in electromagnetic interference (EMI) shielding and other integrated functions for wearable electronics. However, its insufficient change amplitude of resistance with compressive strain generally leads to a degradation of shielding performance during deformation. Here, an innovative loading strategy of conductive materials on polymer foam is proposed to significantly increase the contact probability and contact area of conductive components under compression.
View Article and Find Full Text PDF[Strangulation].
Radiologie (Heidelb)
November 2024
Deutsches Krebsforschungszentrum, Abteilung Radiologie, Heidelberg, Deutschland.
Clinical Problem: Detection of manual strangulation, choking and hanging, as well as the intensity of these actions, helps to clarify the course of events, to enforce the state's right to prosecute and ultimately to protect against further attacks. However, this is complicated by the scarcity of externally visible findings.
Standard Procedure To Date: The forensic examination of the head and neck after strangulation is carried out by means of external inspection to detect injuries and congestion above the strangulation level.
Application of Single Cell Type-Derived Spheroids Generated by Using a Hanging Drop Culture Technique in Various Disease Models: A Narrow Review.
Cells
September 2024
Departments of Ophthalmology, Sapporo Medical University, S1W17, Chuo-ku, Sapporo 060-8556, Japan.
Cell culture methods are indispensable strategies for studies in biological sciences and for drug discovery and testing. Most cell cultures have been developed using two-dimensional (2D) culture methods, but three-dimensional (3D) culture techniques enable the establishment of models that replicate various pathogenic conditions and they provide valuable insights into the pathophysiology of various diseases as well as more precise results in tests for drug efficacy. However, one difficulty in the use of 3D cultures is selection of the appropriate 3D cell culture technique for the study purpose among the various techniques ranging from the simplest single cell type-derived spheroid culture to the more sophisticated organoid cultures.
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