The nucleotide sequence of the complete chloroplast genome of P. jezoensis was completed. The total genome size was 124 146 bp, containing a pair of very short inverted repeats (IRa and IRb) of 422 bp, which were separated by large single copy (LSC) and small single copy (SSC) with 66 956 bp and 56 346 bp, respectively. The overall GC contents of the plastid genome were determined as 38.8%. One hundred fifteen genes including 68 peptide-encoding genes, 35 tRNA genes, four rRNA genes, six open-reading frames, and two pseudogenes were annotated. In these genes, 15 genes contained only one or two introns. Phylogenetic analyses using maximum likelihood (ML) methods were performed from fully sequenced Gymnosperms and other species of dataset composed of 69 protein-coding genes.
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http://dx.doi.org/10.3109/19401736.2015.1079894 | DOI Listing |
Zhongguo Zhong Yao Za Zhi
December 2024
Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
The chloroplast genome is an important tool for studying plant classification, evolution, and the heterologous production of secondary metabolites and protein drugs. With advancements in sequencing technology and reductions in sequencing costs, chloroplast genome data have rapidly accumulated. However, existing chloroplast genome databases suffer from issues such as incomplete data, inadequate management, and inconsistent, inaccurate information, posing significant challenges for the development and utilization of the chloroplast genome.
View Article and Find Full Text PDFThe genus boasts abundant germplasm resources and comprises numerous species. Among these, medicinal plants of this genus, which have a long history, have garnered attention of scholars. This study sequenced and analyzed the chloroplast genomes of six species of medicinal plants (, , , , , and , respectively) to explore their interspecific relationships.
View Article and Find Full Text PDFMitochondrial DNA B Resour
January 2025
Department of Environment and Resource Studies, Mahasarakham University, Maha Sarakham, Thailand.
The complete plastome size of DC. 1813 was 159,893 bp in length and has a typical quadripartite structure. The 87,148-bp-long large single-copy and the 18,763-bp-long small single-copy regions were separated by a pair of inverted repeats (each 26,991 bp).
View Article and Find Full Text PDFMol Biol Evol
January 2025
Department of Molecular Biology, Max Planck Institute for Biology Tübingen, 72076 Tübingen, Germany.
Plant cells have two major organelles with their own genomes: chloroplasts and mitochondria. While chloroplast genomes tend to be structurally conserved, the mitochondrial genomes of plants, which are much larger than those of animals, are characterized by complex structural variation. We introduce TIPPo, a user-friendly, reference-free assembly tool that uses PacBio high-fidelity long-read data and that does not rely on genomes from related species or nuclear genome information for the assembly of organellar genomes.
View Article and Find Full Text PDFSTAR Protoc
January 2025
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
The plastid-encoded RNA polymerase (PEP) plays an essential role in the transcription of the chloroplast genome. Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is fused to an epitope tag. We describe experimental procedures for designing transformation constructs for PEP purification, selection, and analysis of transplastomic tobacco plants.
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