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http://dx.doi.org/10.1002/pmic.201570153 | DOI Listing |
Methods Mol Biol
December 2024
Department of Chemistry, North Carolina State University, Raleigh, NC, USA.
In recent years, discovery proteomics has emerged as a pivotal tool in biological research, especially when studying the intricate relationships among multiple organisms. To delve deeper into these interactions, we pioneered a bottom-up proteomics workflow. Using nanoLC-MS/MS and a label-free quantification method, this work specifically examines the differential protein expression in fleas (Ctenocephalides felis felis) that have been experimentally infected with Bartonella henselae, the causative agent of cat scratch disease (CSD).
View Article and Find Full Text PDFAt present, there are few reports about the proteomics changes provoked by butylated hydroxytoluene (BHT) supplementation on cryopreserved semen in mammals. Thus, we aimed to evaluate the effects of different concentrations of BHT on goat sperm and to investigate the proteomics changes of adding BHT to cryopreserved goat () sperm. Firstly, semen samples were collected from four goats, and frozen in the basic extenders containing different concentrations of BHT (0.
View Article and Find Full Text PDFInt J Mol Sci
August 2022
Department of Endocrinology, Institute of Endocrine and Metabolic Diseases, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, Clinical Research Hospital of Chinese Academy of Sciences (Hefei), University of Science and Technology of China, Hefei 230001, China.
Background And Aims: Non-alcoholic fatty liver disease (NAFLD) affects one-quarter of individuals worldwide. Liver biopsy, as the current reliable method for NAFLD evaluation, causes low patient acceptance because of the nature of invasive sampling. Therefore, sensitive non-invasive serum biomarkers are urgently needed.
View Article and Find Full Text PDFNucleic Acids Res
January 2022
Technical University of Munich, Computational Mass Spectrometry, 85354 Freising, Bavaria, Germany.
ProteomicsDB (https://www.ProteomicsDB.org) is a multi-omics and multi-organism resource for life science research.
View Article and Find Full Text PDFClin Oral Investig
May 2021
Programa de Pós-graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil.
Objectives: The present study aimed to identify proteins obtained from pulp tissue and correlate with each clinical diagnosis (healthy pulp, inflamed pulp, and necrotic pulp).
Materials And Methods: A total of forty-five molars were used. Three biological replicas were evaluated.
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