AI Article Synopsis
- The first complete nucleotide sequence of a tomato spotted wilt virus (TSWV) isolate from the U.S., named PA01, has been reported, showcasing its tripartite genome consisting of L, M, and S RNAs of varying lengths.
- Comparisons of nucleotide and amino acid sequences reveal differences between the PA01 isolate and previously studied TSWV isolates, highlighting its unique characteristics.
- Phylogenetic analysis indicates that PA01 belongs to a different clade than a Hawaiian isolate and shows evidence of genetic reassortment with isolates from South Korea, Italy, and Brazil, suggesting significant genetic exchange among TSWV isolates from different regions.
Article Abstract
We report the first complete nucleotide sequence of a tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae) isolate from the United States. The tripartite genome of PA01 consisted of L, M and S RNAs of 8914, 4765 and 2984 nt, respectively. Similarity percentages in nucleotide and amino acid sequence among PA01 and previously characterized TSWV isolates are provided here. Phylogenetic analysis on the RNA-dependent RNA polymerase (RdRp) gene placed PA01 in a different clade from an isolate from Hawaii that was partially characterized previously. Evidence of two putative reassortment events in the M segment, among PA01 and isolates from South Korea, Italy and Brazil, was found by phylogenetic and recombination analysis, further supporting a role for genetic exchange among isolates of different geographic origin in TSWV evolution.
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