YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560777 | PMC |
| http://dx.doi.org/10.1038/ncomms9041 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Growth of microbes in competitive lifestyles promotes increased ARGs in soil microbiota: insights based on genetic traits.
Microbiome
January 2025
Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Background: The widespread selective pressure of antibiotics in the environment has led to the propagation of antibiotic resistance genes (ARGs). However, the mechanisms by which microbes balance population growth with the enrichment of ARGs remain poorly understood. To address this, we employed microcosm cultivation at different antibiotic (i.
View Article and Find Full Text PDFIon homeostasis and coordinated salt tolerance mechanisms in a barley (Hordeum vulgare L.)doubled haploid line.
BMC Plant Biol
January 2025
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms of Ministry of Agriculture and Rural Affairs (Shanghai), Biotechnology Research Institute of Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China.
Salinization poses a significant challenge in agriculture. Identifying salt-tolerant plant germplasm resources and understanding their mechanisms of salt tolerance are crucial for breeding new salt-tolerant plant varieties. However, one of the primary obstacles to achieving this goal in crops is the physiological complexity of the salt-tolerance trait.
View Article and Find Full Text PDFPloidy levels influence cold tolerance of Cyclocarya paliurus: insight into the roles of WRKY genes.
BMC Genomics
January 2025
College of Forestry and Grassland, Nanjing Forestry University, Nanjing, 210037, China.
Cold stress in winter is one of the most severe abiotic stresses on plant growth and flourishing, and the selection of cold tolerant genotypes is an important strategy to ensure the safety of plant growth and development. Cyclocarya paliurus, a diclinous and versatile tree species originally in subtropical regions, has been introduced and cultivated in the warm temperate zone of China to meet the increasing market demand for its leaf yield. However, information regarding its cold tolerance remains limited.
View Article and Find Full Text PDFSpermine driven water deficit tolerance in early growth phases of sweet corn genotypes under hydroponic cultivation.
Sci Rep
January 2025
Department of Applied Plant Biology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary.
Sweet corn is highly susceptible to water deprivation, making it crucial to identify effective strategies for enhancing its tolerance to water deficit conditions. This study investigates the novel application of Spermine as a bio-stimulant to improve sweet corn (Zea mays L. var.
View Article and Find Full Text PDFTitanium dioxide -mediated regulation of enzymatic and non-enzymatic antioxidants, pigments, and diosgenin content promotes cold stress tolerance in Trigonella foenum-graecum L.
Sci Rep
January 2025
Plant Production Engineering and Genetics Department, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
Abiotic stresses, notably cold stress, significantly influence various aspects of plant development and reproduction. Various approaches have been proposed to counteract the adverse impacts of cold stress on plant productivity. The unique properties of nanoparticles contribute to an enhanced tolerance of plants to challenging conditions.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!