We observed over 400-fold enhanced fluorescence from single Cy5 molecules assembled on multilayer silver nanoparticle-dielectric-metal (PDM) substrate. This substantial enhancement is due to the near-field enhanced excitation, emission, and interaction of Cy5 with plasmonic nanostructures. Experimental observation is supported by finite-element method calculations.
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http://dx.doi.org/10.1039/c5cc03581c | DOI Listing |
Nat Biotechnol
January 2025
Department of Automation, Tsinghua University, Beijing, China.
Super-resolution (SR) neural networks transform low-resolution optical microscopy images into SR images. Application of single-image SR (SISR) methods to long-term imaging has not exploited the temporal dependencies between neighboring frames and has been subject to inference uncertainty that is difficult to quantify. Here, by building a large-scale fluorescence microscopy dataset and evaluating the propagation and alignment components of neural network models, we devise a deformable phase-space alignment (DPA) time-lapse image SR (TISR) neural network.
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Multicolor fluorescent encryption systems that respond to specific stimuli have drawn widespread attention to data storage and encryption due to their low cost and facile data access. However, existing encryption systems are limited by encryption materials, restricting their encryption depth. This study uses DNA molecules as encryption materials that offer exceptional specificity and encryption depth within sequences.
View Article and Find Full Text PDFInt J Pharm
January 2025
The Comprehensive Breast Care Center, The Second Affiliated Hospital of Xi'an Jiaotong University, No.157 Xiwu Road, Xi'an, Shaanxi 710004, China. Electronic address:
Both photothermal therapy (PTT) and chemodynamic therapy (CDT) are designed to focus their antitumor effect on only the tumor site, thereby minimizing unwanted severe damage to healthy tissue outside the tumor. However, each monotherapy is limited in achieving complete tumor eradication, resulting in tumor recurrence. The combination of multiple therapies may help to overcome the limitations of single therapy, improve the chances of complete tumor eradication, and reduce the risk of recurrence.
View Article and Find Full Text PDFInt J Pharm
January 2025
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137 China. Electronic address:
Hepatocellular carcinoma is one of the leading causes of cancer deaths globally and a key hindrance to extending life expectancy. Celastrol (CEL) demonstrates excellent antitumor activity, but faces challenges like low solubility and a narrow therapeutic window, limiting its clinical application. To address these limitations, drug combinations and nano-delivery systems have emerged as effective solutions.
View Article and Find Full Text PDFBiomaterials
January 2025
Lab of Molecular Imaging and Translational Medicine (MITM), Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University & International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and Treatment, Xi'an, Shaanxi, 710126, China. Electronic address:
The secondary near-infrared region (NIR-II) fluorescence imaging-guided photothermal therapy (PTT) offers a noninvasive and light-controllable treatment option for deep-seated cancers. However, the development of NIR-II photothermal agents (NIR-II PTAs) that possess the desired properties of high molar absorption coefficient (ε), fluorescence quantum yield (QY), and photothermal conversion efficiency (PCE) remain a challenge due to the contradiction between radiative and nonradiative processes. Herein, we propose a novel side-chain heteroatom substitution engineering strategy to simultaneously enhance ε, QY, and PCE by modifying the molecular planarity.
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