AI Article Synopsis
- The study aimed to understand how riboflavin permeates and saturates sclera tissue to find an optimal dosage for sclera cross-linking.
- Five human cadaver eyes and 36 New Zealand rabbits were used in experiments, where different concentrations and application times of riboflavin were tested and analyzed using confocal and two-photon microscopy.
- Results showed that riboflavin saturation in the sclera was achieved after 20 minutes, and higher concentrations resulted in greater fluorescence intensity, indicating better absorption.
Article Abstract
Objective: To investigate the permeability and saturation characteristics of riboflavin in sclera tissue, in order to explore a reasonable dosage regimen in sclera cross-linking.
Methods: Experimental study. Five human cadaver eyes were also included in this study, every eye was cut into seven sclera stripes and then 0.1% riboflavin solution was dripped to the sclera stripes for 5, 10, 15, 20, 25, 30 minutes respectively. After that, the stripes were made into frozen sections and observed by confocal microscope. To calculate the mean fluorescence density and compare with each other. In addition, 36 New Zealand rabbits were randomly divided into group A and B groups. Each group was divided into six subgroups. In group A, 0.1% riboflavin solution was dripped to the sclera of right eye for 5, 10, 15, 20, 25 and 30 minutes respectively. In group B, 0.5% ribof lavin solution was dripped to the sclera of right eye for 5, 10, 15, 20, 25 and 30 minutes respectively. The left eye were given with physiological saline as blank check. Executed the rabbits to take out the eye and then the nose upper quadrant of the equator sclera was taken to made into frozen sections which were observed by two-photon microscope. To calculate the mean fluorescence density and compare the six subgroups in every group respectively, and compare the subgroups with the same riboflavin-applicated time between group A and B respectively.
Results: The fluorescence intensity of sclera gradually strengthened with the longer riboflavin-applicated time, but there was no statistical difference between 20 minutes and the longer time groups (P > 0.05). With the same riboflavin-applicated time in rabbit sclera, the fluorescence intensity of 0.5% concentration group was higher than 0.1% concentration group (P < 0.05).
Conclusions: The riboflavin reached an saturation level in the sclera of human cadaver eyes and rabbit eyes after 20 minutes application. The higher concentration of riboflavin-applicated, the higher concentration of riboflavin in the rabbit sclera.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Analysis and optimization of the landing zone parameters of a sclera lens model.
Arq Bras Oftalmol
January 2025
Ophthalmology and Visual Sciences Department, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Purpose: This study aimed to modify scleral contact lenses to achieve a desired compression standard and to evaluate the effectiveness and reliability of the adjustments.
Methods: In this nonrandomized, noncomparative, and partially masked study Scleral contact lens fittings were analyzed in 20 eyes of 12 patients (50% women, 50% men) diagnosed with keratoconus. Participants were selected based on their need for scleral contact lenses (SCLs), which was determined in complete ophthalmological examinations.
The swinging sclera technique for evisceration.
Orbit
January 2025
Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at University of Texas Health Science Center, Houston, Texas, USA.
Purpose: To present a modified evisceration technique with a full-thickness horizontal sclerotomy and assess post-operative motility and long-term outcomes.
Methods: This is a retrospective chart review of patients who underwent evisceration with a single surgeon (TJM). The standard initial steps of evisceration were performed.
The Ocular Surface and the Anterior Segment of the Eye in the Pseudoexfoliation Syndrome: A Comprehensive Review.
Int J Mol Sci
January 2025
NDDH, Royal Devon University Healthcare NHS Foundation Trust, Barnstaple EX31 4JB, UK.
Pseudoexfoliation syndrome (PXS) is an age-related fibrillopathy where fibrillar exfoliation material accumulates and deposits in ocular and extra-ocular tissue. Within the eye, this substance accumulates on the ocular surface and in the anterior segment of the eye, impacting ocular structures such as the conjunctiva, Tenon's capsule, sclera, cornea, iris, ciliary body, trabecular meshwork, and lens. This review aims to collate the current literature on how each anatomical part of the eye is affected by PXS, with a strong focus on molecular changes.
View Article and Find Full Text PDFSilicone Fiducial Markers Improve Precision in Uveal Melanoma Radiation Therapy.
Cancers (Basel)
January 2025
Department of Ophthalmology, University of Lübeck, University Medical Center Schleswig-Holstein, Campus Lübeck, 23562 Lübeck, Germany.
: Accurate target definition, treatment planning and delivery increases local tumor control for radiotherapy by minimizing collateral damage. To achieve this goal for uveal melanoma (UM), tantalum fiducial markers (TFMs) were previously introduced in proton and photon beam radiotherapy. However, TFMs cause pronounced scattering effects in imaging that make the delineation of small tumors difficult.
View Article and Find Full Text PDFTransconjunctival correction of involutional entropion: A video demonstration.
Indian J Ophthalmol
February 2025
Department of Oculoplasty and Oncology Services (Dr. Rajendra Prasad Centre for Ophthalmic Sciences), AIIMS, New Delhi, India.
Background: Involution or aging is the most common cause of lower eyelid entropion (in-turning of eyelid margin) in the elderly population. Various pathomechanisms have been postulated for its occurrence. Aging leads to laxity of tissues and loss of muscle tone.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!