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| http://dx.doi.org/10.5152/AnatolJCardiol.2015.6285 | DOI Listing |
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The umbrella cell keratin network: organization as a tile-like mesh, formation of a girded layer in response to bladder filling, and dependence on the plectin cytolinker.
bioRxiv
June 2024
Department of Medicine Renal-Electrolyte Division and George M. O'Brien Pittsburgh Center for Kidney Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
The keratin cytoskeleton and associated desmosomes contribute to the mechanical stability of epithelial tissues, but their organization in bladder umbrella cells and their responses to bladder filling are poorly understood. Using super-resolution confocal microscopy, along with 3D image reconstruction and platinum replica electron microscopy, we observed that the apical keratin network of umbrella cells was organized as a dense tile-like mesh comprised of tesserae bordered on their edges by cortical actin filaments, filled with woven keratin filaments, and crosslinked by plectin. A band of keratin was also observed at the cell periphery that was linked to the junction-associated actin ring by plectin.
View Article and Find Full Text PDFEffectiveness of a new endodontic irrigation system for removing smear layer and dissolving simulated organic matter.
Clin Oral Investig
December 2023
Department of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo - USP, Al. Octávio Pinheiro Brizolla 9-75, Vila Universitária Bauru, Bauru, SP, CEP: 17012-901, Brazil.
Objectives: The study aimed to evaluate the potential for the dissolution of organic tissue in areas of simulated complexity and cleaning of root canal walls of the new iVac® endodontic irrigation system.
Material And Methods: Thirty mandibular premolars were evaluated by scanning electron microscopy before and after biomechanical preparation. Then, they were distributed according to the final irrigation protocol into groups with conventional irrigation, ultrasonic agitation with metallic insert (UA), and iVac® system, and new photomicrographs were obtained.
The h-current controls cortical recurrent network activity through modulation of dendrosomatic communication.
bioRxiv
July 2023
Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06510; Institute of Neuroscience, University of Oregon, Eugene, OR 97403.
A fundamental feature of the cerebral cortex is the ability to rapidly turn on and off maintained activity within ensembles of neurons through recurrent excitation balanced by inhibition. Here we demonstrate that reduction of the h-current, which is especially prominent in pyramidal cell dendrites, strongly increases the ability of local cortical networks to generate maintained recurrent activity. Reduction of the h-current resulted in hyperpolarization and increase in input resistance of both the somata and apical dendrites of layer 5 pyramidal cells, while strongly increasing the dendrosomatic transfer of low (<20 Hz) frequencies, causing an increased responsiveness to dynamic clamp-induced recurrent network-like activity injected into the dendrites and substantially increasing the duration of spontaneous Up states.
View Article and Find Full Text PDFInvestigation of the effect of hydrochloric acid with different concentrations on mineral trioxide aggregate plug and dentin.
Dent Res J (Isfahan)
May 2023
Department of Endodontic, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Background: Mineral trioxide aggregate (MTA) becomes a hard mass after setting and making it difficult to remove and can cause significant problems in the retreatment process. The aim of this study was to evaluate the effect of different concentrations of hydrochloric acid (HCl) on MTA dissolution and its effect on dentin.
Materials And Methods: In this study, 45 single-root premolars were selected.
Analysis of porosity, sealer dissolution and apical extrusion of endodontic sealers: A micro computed tomography study.
J Oral Biol Craniofac Res
June 2023
Department of Conservative Dentistry and Endodontics, King George Medical University, Lucknow, India.
Aim: The aim of the study was to determine the porosity, dissolution and apical extrusion of AH Plus, MTA Fillapex and EndoSequence BC sealer after immersion in phosphate buffered saline (PBS) using microcomputed tomography (micro-CT).
Methodology: Forty-eight single-rooted teeth were selected. Gutta percha and one of the aforementioned root canal sealers was used for obturation using continuous wave technique.
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