Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528169 | PMC |
http://dx.doi.org/10.3389/fmicb.2015.00810 | DOI Listing |
Front Microbiol
January 2025
UPR4301 Centre de Biophysique Moléculaire (CBM), Orléans, France.
We investigated the metabolome of the iron- and sulfur-oxidizing, extremely thermoacidophilic archaeon grown on mineral pyrite (FeS). The extraction of organic materials from these microorganisms is a major challenge because of the tight contact and interaction between cells and mineral materials. Therefore, we applied an improved protocol to break the microbial cells and separate their organic constituents from the mineral surface, to extract lipophilic compounds through liquid-liquid extraction, and performed metabolomics analyses using MALDI-TOF MS and UHPLC-UHR-Q/TOF.
View Article and Find Full Text PDFCommun Biol
January 2025
Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
CRISPR-Cas9 genome editing has been extensively applied in both academia and clinical settings, but its genotoxic risks, including large insertions (LgIns), remain poorly studied due to methodological limitations. This study presents the first detailed report of unintended LgIns consistently induced by different Cas9 editing regimes using various types of donors across multiple gene loci. Among these insertions, retrotransposable elements (REs) and host genomic coding and regulatory sequences are prevalent.
View Article and Find Full Text PDFCureus
December 2024
Surgery, Norfolk and Norwich University Hospital, Norwich, GBR.
Surgeon fatigue significantly affects cognitive and motor functions, increasing the risk of errors and adverse patient outcomes. Traditional fatigue management methods, such as structured breaks and duty-hour limits, are insufficient for real-time fatigue detection in high-stakes surgeries. With advancements in artificial intelligence (AI), there is growing potential for AI-driven technologies to address this issue through continuous monitoring and adaptive interventions.
View Article and Find Full Text PDFBiomedicines
November 2024
Department of Internal Medicine II, University Hospital Regensburg, 93053 Regensburg, Germany.
While your nightly symphony may be testing your loved one's patience, it could also be giving your own heart reasons to complain [...
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!