Skin plays a protective role against the loss of water and external aggression, including mechanical stresses. These crucial functions are ensured by different cutaneous layers, particularly the stratum corneum (SC). During aging, the human skin reveals some apparent modifications of functionalities such as a loss of elasticity. Our investigations aimed at demonstrating that Raman microspectroscopy, as a label-free technique with a high molecular specificity, is efficient to assess in vivo the molecular composition of the skin and the alterations underwent during aging. Our approach was based on a search for correlation between Raman data collected on healthy female volunteers of different ages (from 21 to 70 years old) by means of a remote confocal Raman and skin firmness measurements used as a reference method. Raman and biometric data were then submitted to a partial least square (PLS)-based data processing. Our experiments demonstrated the potential of Raman microspectroscopy to provide an objective in vivo assessment of the skin "biological age" that can be very different from the "chronological age" of the person. In addition, Raman features sensitive to the elasticity and the fatigability of the SC were highlighted. Thereafter, calibration transfer functions were constructed to show the possibility to compare the results obtained during two distinct measurement campaigns conducted with two Raman probes of the same conception. This approach could lead to several interesting prospects, in particular by objectifying the effects of dermocosmetic products on the superficial layers of the skin and by accessing some underlying molecular mechanisms.
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Mar Pollut Bull
January 2025
Faculty of Marine Resources and Environment, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-Ku, Tokyo 108-8477, Japan. Electronic address:
Microplastic pollution in marine environments poses significant environmental risks due to its widespread presence. Traditional micro-imaging measurement of microplastics often rely on post-cruise laboratory analyses. In this study, we explored the feasibility of onboard microplastic measurement using Raman spectroscopy, with a focus on polyethylene (PE).
View Article and Find Full Text PDFFood Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
UMR SayFood 0782, Université Paris-Saclay, INRAE, Palaiseau, AgroParisTech, France.
Assessing the contamination of paper and board (P&B) food packaging materials poses significant challenges due to the sensitivity limits of analytical methods and the low precision of sampling processes. This study aims to enhance the understanding of P&B food packaging contamination by investigating the distribution of contaminants at different scales using a combination of chromatographic and spectroscopic techniques. A total of 36 substances were targeted, including phthalates, photoinitiators, and bisphenol A.
View Article and Find Full Text PDFJ Phys Chem Lett
January 2025
Biomolecular Physics Department, Faculty of Physics, Babeş-Bolyai University, 1 M. Kogalniceanu Str., 400084 Cluj-Napoca, Romania.
Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool for analyzing nucleic acids due to its exceptional sensitivity and specificity. This study rigorously investigates not only the impact of polyA strands of different lengths (, 5, 10, 15, and 20 adenine bases) but also their distinct grafting strategy (SH at 5' and NH at 5' end) on the SERS signal of DNA strand using synthesized gold nanoparticles (AuNPs) on graphene oxide sheets (GO-AuNPs). By comparing the thiol vs amine bonding onto the GO-AuNP nanoplatform, we found a strong correlation between the adenine peak intensity at 732 cm and the strand length for both grafting methods (SH at 5' end or NH at 5' end).
View Article and Find Full Text PDFEnviron Int
January 2025
Center for Reproductive Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. Electronic address:
Microplastics (MPs) are pervasive environmental contaminants, resulting in unavoidable human exposure. This study identified MPs in follicular fluid and investigated the specific MPs and mechanisms that adversely affect oocytes. MPs in the follicular fluid of 44 infertile women undergoing assisted reproductive technology were measured using Raman microspectroscopy.
View Article and Find Full Text PDFACS Appl Mater Interfaces
December 2024
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokko, Nada Kobe 657-8501, Japan.
Manufacturing using adhesion technology has attracted much attention. Examples of adhesion include the lay-up of carbon fiber reinforced thermoplastic prepregs and the lamination of food packaging. In single-component adhesion systems, the analysis of the boundary region poses challenges because of the absence of chemical and physical discrimination at the adhesion interphase.
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