AI Article Synopsis
- The study focused on measuring metabolic activity, pH levels, and oxygen concentration in vitro using chicken spleen slices, employing both real-time sensor data and numerical simulations.
- A finite element method model was created to represent biochemical processes; by comparing simulated results to real data, researchers determined spatiotemporal values of key metabolic indicators.
- Findings revealed that sensor placement significantly affects metabolic readings, and the vitality of tissue samples is highly dependent on their size; the research also addresses the model's advantages and limitations.
Article Abstract
We estimated the dynamic cell metabolic activity and the distribution of the pH value and oxygen concentration in tissue samples cultured in vitro by using real-time sensor records and a numerical simulation of the underlying reaction-diffusion processes. As an experimental tissue model, we used chicken spleen slices. A finite element method model representing the biochemical processes and including the relevant sensor data was set up. By fitting the calculated results to the measured data, we derived the spatiotemporal values of the pH value, the oxygen concentration and the absolute metabolic activity (extracellular acidification and oxygen uptake rate) of the samples. Notably, the location of the samples in relation to the sensors has a great influence on the detectable metabolic rates. The long-term vitality of the tissue samples strongly depends on their size. We further discuss the benefits and limitations of the model.
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