The aim of this study was to examine the lysozyme activity in saliva of pharmaceutic industry workers in whom pathologic changes in the oral cavity were previously diagnosed. The study was carried out in the Pharmaceutic Plant "Polfa" in Stargard Gdański at the departments of sulphonamides, acetylsalicylic acid and various syntheses. The study comprised 94 workers from whom resting saliva samples were collected 2-3 hours after breakfast. The lysozyme activity was determined using the modified Litwack method, while proteins were examined using the Lawry method. Our examinations have demonstrated a higher enzyme activity in pharmaceutic industry workers as compared to the control group. The highest lysozyme activity was recorded at the sulphonamides department.
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Vet Immunol Immunopathol
January 2025
Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institute, Zhanjiang, China. Electronic address:
Vibrio alginolyticus is a serious aquaculture bacterial pathogen, which is widely distributed in the ocean and rivers, and cause vibriosis in aquaculture. Therefore, it is imperative to develop effective vaccine to prevent vibriosis. In this study, the efficacy of gr deletion strain (Δgr) of V.
View Article and Find Full Text PDFUnlabelled: The complement cascade is a front-line defense against pathogens. Complement activation generates the membrane attack complex (MAC), a 10-11 nm diameter pore formed by complement proteins C5b through C8 and polymerized C9. The MAC embeds within the outer membrane of Gram-negative bacteria and displays bactericidal activity.
View Article and Find Full Text PDFHeliyon
January 2025
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand.
The study, herein, investigated the effects of the inclusion of longan peel (LP) powder in the diet of Nile Tilapia (), focusing on comparative evaluations of growth performance, immunity, and immune-antioxidant related gene expressions. For this purpose, a total of 300 healthy fish (average initial weight: 13.70 ± 0.
View Article and Find Full Text PDFMolecules
January 2025
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
Macromolecule bactericides present challenges such as low biocompatibility and not being biodegradable, so broad-spectrum bactericides without accumulated bacteria resistance are now in urgent demand all over the world. Lysozyme, a kind of wide-spread natural enzyme easily extracted from nature, has become attractive for agriculture and medicine use. However, Gram-negative bacterial strains are highly resistant to natural lysozymes, which limits their practical application.
View Article and Find Full Text PDFAntioxidants (Basel)
January 2025
School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, Thailand.
Due to its lack of the L-gulonolactone oxidase () enzyme, Nile tilapia is unable to synthesize vitamin C; thus, it requires an adequate level of exogenous vitamin C in its diet. To enhance antioxidant properties and vitamin C-related effects, we employed recombinant technology to integrate the -encoding gene into the chromosome. In this study, fish were divided into four groups: those fed with a basal diet (CON), a basal diet + vitamin C (VC), a basal diet + wild-type (BS), and a basal diet + recombinant (BS+GULO).
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