Our precious studies showed that delipidized water-saline extract from roots of Arum maculatum (AM) contained lectin-phytospermoagglutinins (PSA), which caused agglutination, manifested by the type of a tail with a tail and a head with a tail. This extract we conjugated with fluorescein-isothiocyanate and by means of the conjugate we studied the localization of receptor molecules for PSA on the plasmalemma of human spermatogenic cells and human spermatozoa, obtained from the testis, epididymis and ejaculate. It was established that receptor molecules for PSA were distributed irregularly on the plasmalemma of spermatozoa, that the basic part of these molecules were synthesized on spermatogenic cells and that ejaculate spermatozoa adsorbed such molecules from the sperm plasma.
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Zhongguo Zhen Jiu
January 2025
College of Acupuncture-Moxibustion and Tuina, Chengdu University of TCM, Chengdu 610075, Sichuan Province, China; Second Clinical College, Shanxi University of CM, Jinzhong 030619.
Objective: To observe the effects of the "Zhibian" (BL54)-toward-"Shuidao" (ST28) acupuncture on key regulatory factors during mitochondrial apoptosis of testicular tissue in asthenozoospermia mice, and explore the potential mechanism of the protective effect of acupuncture on reproductive function.
Methods: Thirty C57BL/6 male mice were randomly divided into a blank group, a model group and an acupuncture group, 10 mice in each group. In the model and the acupuncture groups, the intraperitoneal injection of cyclophosphamide (30 mg•kg•d) was delivered for 7 days to prepare the asthenozoospermia model.
Elife
January 2025
Biology of the Testis (BITE) Laboratory, Genetics, Reproduction and Development (GRAD) Research Group, Vrije Universiteit Brussel, Brussels, Belgium.
Although the impact of gender-affirming hormone therapy (GAHT) on spermatogenesis in trans women has already been studied, data on its precise effects on the testicular environment is poor. Therefore, this study aimed to characterize, through histological and transcriptomic analysis, the spermatogonial stem cell niche of 106 trans women who underwent standardized GAHT, comprising estrogens and cyproterone acetate. A partial dedifferentiation of Sertoli cells was observed, marked by the co-expression of androgen receptor and anti-Müllerian hormone which mirrors the situation in peripubertal boys.
View Article and Find Full Text PDFAntioxidants (Basel)
November 2024
School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan.
Cisplatin (CIS) is a widely used chemotherapeutic agent, but its side effects, such as oxidative stress, inflammation, and apoptosis, often lead to male reproductive damage. Oxidative stress, primarily caused by the excessive generation of reactive oxygen species (ROS), plays a critical role in disrupting testicular homeostasis, resulting in spermatogenic impairment and tissue injury. L-cysteine (CYS), a semi-essential amino acid with potent antioxidant and anti-inflammatory properties, may offer protection against CIS-induced oxidative damage.
View Article and Find Full Text PDFArch Med Res
January 2025
Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey. Electronic address:
Aim: Nowadays, the electromagnetic field (EMF) has become an issue of electromagnetic pollution. This study aimed to determine the effect of 5 G Fr1 frequency band EMF waves on endoplasmic reticulum (ER) stress in testicular tissue and to demonstrate the efficacy of coenzyme Q10 (CoQ10) in suppressing the potential situation.
Materials And Methods: Three groups of eight male Sprague-Dawley rats were established.
Zool Res
January 2025
Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong 510623, China. E-mail:
The proteasome, an evolutionarily conserved proteolytic complex comprising the 20S core particle and 19S regulatory particles, performs both shared and distinct functions across various tissues and organs. Spermatogenesis, a highly complex developmental process, relies on proteasome activity at multiple stages to regulate protein turnover. In this study, we selected the 20S subunit PSMA1 and 19S regulatory subunit PSMD2 to investigate the potential functions of the proteasome in spermatogenesis.
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