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Improvement of heme oxygenase-1-based heme sensor for quantifying free heme in biological samples. | LitMetric

Improvement of heme oxygenase-1-based heme sensor for quantifying free heme in biological samples.

Anal Biochem

Department of Bioscience and Bioinformatics, Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Kawazu, Iizuka 820-8502, Japan. Electronic address:

Published: November 2015

AI Article Synopsis

Article Abstract

We recently reported a novel heme sensor using fluorescently labeled heme oxygenase-1; however, its inherent enzyme activity would be a potential obstacle in quantifying heme in biological samples. Here, we found that mutation of the catalytically important residue, Asp140, with histidine in the sensor not only diminished the heme degradation activity but also increased heme binding affinity. The sensor with a visible fluorophore was also found to be beneficial to avoid background emission from endogenous substance in biological samples. By using the improved heme sensor, we succeeded in quantifying free heme in rat hepatic samples for the first time.

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Source
http://dx.doi.org/10.1016/j.ab.2015.08.004DOI Listing

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