AI Article Synopsis
- Industrial yeasts like the sake yeast strain Kyokai no. 7 (K7) typically can't sporulate, but treatment with rapamycin restores this ability, indicating active TORC1 during sporulation.
- Using various assays, researchers observed that while RPS26A transcription was repressed and Npr1 was dephosphorylated—pointing to lost TORC1 function—some nitrogen catabolite repression-sensitive genes remained uninduced and Cln3 levels stayed constant.
- Overall, they found that TORC1 activity is partially reduced during nitrogen starvation in K7 cells, suggesting a complex regulation under stress conditions.
Article Abstract
Industrial yeasts are generally unable to sporulate but treatment with the immunosuppressive drug rapamycin restores this ability in a sake yeast strain Kyokai no. 7 (K7), Saccharomyces cerevisiae. This finding suggests that TORC1 is active under sporulation conditions. Here, using a reporter gene assay, Northern and Western blots, we tried to gain insight into how TORC1 function under nitrogen starvation conditions in K7 cells. Similarly to a laboratory strain, RPS26A transcription was repressed and Npr1 was dephosphorylated in K7 cells, indicative of the expected loss of TORC1 function under nitrogen starvation. The expression of nitrogen catabolite repression-sensitive genes, however, was not induced, the level of Cln3 remained constant, and autophagy was more slowly induced than in a laboratory strain, all suggestive of active TORC1. We conclude that TORC1 activity is partially reduced under nitrogen starvation conditions in K7 cells.
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| http://dx.doi.org/10.1016/j.jbiosc.2015.07.002 | DOI Listing |
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