HPLC Method for Simultaneous Quantification of Bakuchiol and Minor Furocoumarins in Bakuchiol Extract from Psoralea corylifolia.

A simple, sensitive, and rapid HPLC method was developed to analyze bakuchiol and two furocoumarins (psoralen and angelicin) simultaneously in bakuchiol extracts from Psoralea corylifolia seeds. The analysis was performed within 30 min on a phenyl-hexyl column using gradient elution with a mobile phase composed of water and methanol with UV detection at 260 nm for bakuchiol and 246 nm for psoralen and angelicin. The method was validated with respect to linearity (r2>0.99 for all components), accuracy (>95% for all components), and precision (<2% RSD for both interday and intraday). Sensitivity of impurity detection in the sample was achieved as low as 0.36 and 0.31 μg/mL for psoralen and angelicin, respectively. Therefore, the method is suitable for QC of P. corylifolia extracts and bakuchiol related samples.