Bioluminescence resonance energy transfer (BRET) is a well-established technique for investigating G protein-coupled receptor (GPCR) pharmacology. BRET enables the monitoring of molecular proximity through the use of heterologously expressed proteins of interest and/or fluorophore-labeled ligands. Fusion to a donor luciferase enzyme or an acceptor fluorophore and subsequent detection of resonance energy transfer indicate the close proximity of the molecules of interest. As BRET is readily applied to the study of numerous GPCR signaling and regulatory paths, it is an ideal technique for investigating the pharmacology of biased ligands and receptors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-2914-6_13 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nanoparticle-mediated light-driven LAMP combined with test strips for sensitive and rapid visual detection of antibiotic resistance genes.
J Hazard Mater
December 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:
Antibiotic resistance genes (ARGs) are markers of drug-resistant pathogens, monitoring them contributes to prevent resistance to drugs. The detection methods for ARGs including PCR and isothermal amplification are sensitive and selective. However, it may take several hours or cannot be used on spot.
View Article and Find Full Text PDFTwo-electron two-nucleus effective Hamiltonian and the spin diffusion barrier.
Sci Adv
January 2025
Institute of Molecular Physical Science, ETH Zurich, 8093 Zurich, Switzerland.
Dynamic nuclear polarization (DNP) and emerging quantum technologies rely on the spin transfer in electron-nuclear hybrid quantum systems. Spin transfers might be suppressed by larger couplings, e.g.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
USC Keck School of Medicine, Los Angeles, CA, USA.
Background: Human Apolipoprotein (APOE) has three isoforms, ε2, ε3, and ε4 among which ε4 (APOE4) confers the highest risk for late-onset Alzheimer's disease (AD). APOE4 is also the most prone to aggregate among APOE isoforms. Current evidence strongly suggests that APOE aggregation leads to neuronal dysfunction and eventually to AD.
View Article and Find Full Text PDFBasic Science and Pathogenesis.
Alzheimers Dement
December 2024
Purdue University, West Lafayette, IN, USA.
Background: Exposure to environmental chemicals such as lead (Pb) during vulnerable developmental periods and even in adult stage can result in adverse health outcomes later in life. Human cohort studies have demonstrated associations between Pb exposure and Alzheimer's Disease (AD) onset in later life which were further corroborated by findings from animal studies. The molecular pathway linking Pb exposure and increased AD risk, however, remains elusive.
View Article and Find Full Text PDFRobust Quantum Control via Multipath Interference for Thousandfold Phase Amplification in a Resonant Atom Interferometer.
Phys Rev Lett
December 2024
Department of Physics and Astronomy and Center for Fundamental Physics, Northwestern University, Evanston, Illinois 60208, USA.
We introduce a novel technique for enhancing the robustness of light-pulse atom interferometers against the pulse infidelities that typically limit their sensitivities. The technique uses quantum optimal control to favorably harness the multipath interference of the stray trajectories produced by imperfect atom-optics operations. We apply this method to a resonant atom interferometer and achieve thousandfold phase amplification, representing a 50-fold improvement over the performance observed without optimized control.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!