[EVALUATION OF FUNCTIONAL FEATURES AND STRESS RESISTANCE OF ISOGENIC TOXIGENIC AND NON-TOXIGENIC BIOVAR EL TOR VIBRIO CHOLERAE STRAINS].

Aim: Comparative evaluation of functional features of toxigenic biovar El Tor Vibrio cholerae strains and their spontaneous non-toxigenic mutants and study of their resistance to saline and oxidative stress.

Materials And Methods: 8 biovar El Tor V. cholerae strains were studied: 4 clinical strains isolated in 1970 from patients in Astrakhan and 4 spontaneous non-toxigenic mutants of these strains that have lost cholera toxin genes as a result of residence in river water at the temperature of 25°C. Protein composition was determined in polyacrylamide gel electrophoresis by Laemmli U.K. Stress resistance of the strains was studied by adding H2O2 to 20 mM concentration and NaCl to 3 mM concentration to the cultural suspension.

Results: Loss of cholera toxin genes was shown to be accompanied by changes in the level of expression of 17 proteins including those that take part in energy metabolism, glucose transport, chemotaxis and purine bases. Moreover, non-toxigenic strains were established to be 5-15 times more resistant to saline and oxidative stress compared with toxigenic strains.

Conclusion: Non-toxigenic V. cholerae mutants adopt better to stress factors, therefore the loss of cholera toxin gene in water environment could be one of the methods of adaptation of pathogenic bacteria to changes in the environment.