AI Article Synopsis
- A new HPLC method was developed to analyze trigonelline, caffeine, chlorogenic acid, and related compounds simultaneously using an InertSustain C18 column with specific mobile phase conditions.
- The optimal mobile phase consisted of 0.1% phosphoric acid, 4 mM octanesulfonate, and 15% methanol at a temperature of 35°C.
- In the analysis of ten instant coffee samples, all compounds except theophylline were detected, revealing a trend where higher caffeine levels correlated with lower trigonelline and chlorogenic acid levels, and a strong correlation (R(2) = 0.887) was found between trigonelline and chlorogenic acid contents.
Article Abstract
In order to analyze trigonelline, caffeine, chlorogenic acid, and their related compounds simultaneously, an HPLC method using an InertSustain C18 column and a mobile phase containing octanesulfonate as an ion-pairing reagent under an acidic condition was developed. The optimum mobile phase conditions were determined to be 0.1% phosphoric acid, 4 mM octanesulfonate, and 15% methanol at 35°C. Using the proposed method, trigonelline, nicotinic acid, caffeine, theophylline, chlorogenic acid, and caffeic acid in ten instant coffee samples were analyzed. These analytes except for theophylline were detected in all samples. An increase in the caffeine content in instant coffee samples tended to decrease in both trigonelline and chlorogenic acid contents, and the trigonelline content was found to be correlated well with the chlorogenic acid content (R(2) = 0.887).
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| http://dx.doi.org/10.2116/analsci.31.831 | DOI Listing |
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