AI Article Synopsis
- - Recent advancements in regenerative medicine focus on repairing and improving damaged articular cartilage, and this study explores how flow-induced shear stress affects chondrocytes encapsulated in alginate within a perfusion bioreactor.
- - The study utilized computational fluid dynamics to predict shear stress on cells, leading to the growth of chondrocyte pellets that were cultured under dynamic conditions, resulting in constructs that maintained their shape and showed homogenous cell distribution.
- - Histological and immunohistochemical analyses indicated that the engineered tissue exhibited characteristics typical of natural cartilage, including the presence of type II collagen, suggesting that the growth environment promoted by shear stress supports effective cell growth and tissue regeneration.
Article Abstract
In the recent years, there has been considerable development in the regenerative medicine, which aims to repair, regenerate, and improve injured articular cartilage. The aim of the present study was to investigate the effect of flow-induced shear stress in perfusion bioreactor on alginate encapsulating chondrocytes. The shear stress imposed on the cells in the culture chamber of bioreactor was predicted with computational fluid dynamic. Bovine nasal chondrocytes were isolated and expanded to obtain a pellet. The cell pellet was resuspends in alginate solution, transferred to the culture chamber, and dynamically cultured under direct perfusion. At the end of culture, tissue constructs were examined histologically and by immunohistochemistry. The results of computational fluid dynamic modeling revealed that maximum wall shear stress was 4.820 × 10(-3) Pascal. Macroscopic views of the alginate/chondrocyte beads suggested that it possessed constant shape but were flexible. Under inverted microscope, round shape of chondrocyte observed. Cell distribution was homogeneous throughout the scaffold. Tissue construct subjected to shear showed morphological features, which are characteristic for natural cartilage. Immunohistochemistry results revealed immunopositivity for type II collagens in tissue constructs samples. Flow induced shear stress in the perfusion bioreactor and chnondrocyte encapsulation provide environment to support cell growth, and tissue regeneration and improve cartilage like tissue fabrication.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10561-015-9529-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Variations in humeral and femoral strains across body sizes and limb posture in American alligators.
J Exp Biol
December 2024
Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA.
Bone loading is a crucial factor that constrains locomotor capacities of terrestrial tetrapods. To date, limb bone strains and stresses have been studied across various animals, with a primary emphasis on consistent bone loading in mammals of different sizes and variations in loading regimes across different clades and limb postures. However, the relationships between body size, limb posture and limb bone loading remain unclear in animals with non-parasagittally moving limbs, limiting our understanding of the evolution of limb functions in tetrapods.
View Article and Find Full Text PDFInnovative Dual Combination Cospray-Dried Rock Inhibitor/l-Carnitine Inhalable Dry Powder Aerosols.
ACS Bio Med Chem Au
December 2024
The University of Arizona College of Pharmacy, Skaggs Pharmaceutical Sciences Center, Tucson, Arizona 85721, United States.
This study introduces novel cospray-dried (Co-SD) formulations of simvastatin, a Nrf2 activator ROCK inhibitor, with l-carnitine as molecular mixtures in various molar ratios for targeted pulmonary inhalation aerosol delivery in pulmonary hypertension, optimized for excipient-free dry powder inhalers (DPIs). The two components were spray-dried at various molar ratios by using different starting feed solution concentrations and process parameters. In addition to comprehensive physicochemical characterization, in vitro aerosol dispersion performance as DPIs using two FDA-approved DPI devices with different shear stress properties, in vitro viability as a function of dose on 2D human pulmonary cellular monolayers and on 3D small airway epithelia human primary cultures at the air-liquid interface (ALI), and in vitro transepithelial electrical resistance (TEER) at the ALI were conducted.
View Article and Find Full Text PDFA multiphysics hybrid continuum - agent-based model of in vitro vascularized organoids.
Comput Biol Med
December 2024
Escuela Técnica Superior de Ingeniería, Universidad de Sevilla, Spain. Electronic address:
Background: Organoids are 3D in vitro models that fulfill a hierarchical function, representing a small version of living tissues and, therefore, a good approximation of cellular mechanisms. However, one of the main disadvantages of these models is the appearance of a necrotic core due to poor vascularization. The aim of this work is the development of a numerical framework that incorporates the mechanical stimulation as a key factor in organoid vascularization.
View Article and Find Full Text PDFThe hemodynamic responses to enhanced external counterpulsation therapy in post-PCI patients with a multi-dimension 0/1D-3D model.
J Biomech
December 2024
Department of Cardiology, The Eighth Affiliated Hospital of Sun Yat-sen University, Shenzhen, Guangdong 518033, China; Guangdong Innovative Engineering and Technology Research Center for Assisted Circulation, Sun Yat-sen University, Shenzhen, Guangdong 518033, China. Electronic address:
Enhanced external counterpulsation (EECP) is widely utilized in rehabilitating patients after percutaneous coronary intervention (PCI) and has demonstrated efficacy in promoting cardiovascular function recovery. Although the precise mechanisms of the therapeutic effects remain elusive, it is widely postulated that the improvement of biomechanical environment induced by EECP plays a critical role. This study aimed to unravel the underlying mechanism through a numerical investigation of the in-stent biomechanical environment during EECP using an advanced multi-dimensional 0/1D-3D coupled model.
View Article and Find Full Text PDFCitrate-Coated Iron Oxide Nanoparticles Facilitate Endothelialization of Left Ventricular Assist Device Impeller for Improved Antithrombogenicity.
Adv Sci (Weinh)
December 2024
Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.
Although left ventricular assist devices (LVADs) are an alternative to heart transplantation, their artificial surfaces often lead to serious thrombotic complications requiring high-risk device replacement. Coating blood-contacting surfaces with antithrombogenic endothelial cells is considered an effective strategy for preventing thrombus formation. However, this concept has not yet been successfully implemented in LVADs, as severe cell loss is to be expected, especially on the impeller surface with high prothrombogenic supraphysiological shear stress.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!