AI Article Synopsis
- - We studied mutations in 140 multidrug-resistant tuberculosis (MDR-TB) strains from China, focusing on three genes: rpsL, rrs, and gidB, and found that 65.7% were resistant to streptomycin (STR), mainly from the Beijing strain.
- - The study showed a strong correlation between STR resistance and the Beijing family of tuberculosis (P=0.00) and highlighted that specific mutations in rpsL and rrs were common in STR-resistant isolates.
- - Detecting mutations in these genes had high effectiveness, with 94.6% sensitivity and 91.7% specificity, making them reliable indicators for STR resistance, which could aid the development of rapid
Article Abstract
We investigated the spectrum and frequency of mutations in rpsL, rrs, and gidB among 140 multidrug-resistant tuberculosis (MDR-TB) clinical isolates from China. The association between mutations and different genotypes was also analyzed. Our data revealed that 65.7% of MDR-TB were resistant to streptomycin (STR), and 90.2% of STR-resistant isolates were Beijing strains. STR resistance was correlated with Beijing family (P=0.00). Compared with phenotypic data, detection of mutations for the combination of these 3 genes exhibited 94.6% sensitivity, 91.7% specificity, and 93.6% accuracy. The most common mutations in STR-resistant isolates were rpsL128, 262, and rrs514, of which rpsL128 showed association with Beijing lineage (P=0.00). A combination of these 3 mutations can serve as the reliable predictors for STR resistance, showing the sensitivity, specificity, and accuracy of 85.9%, 97.9%, and 90.0%, respectively. Furthermore, gidBA276C, not A615G, was Beijing lineage specific. These findings are useful to develop rapid molecular diagnostic methods for STR resistance in China.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2015.06.020 | DOI Listing |
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