More than 600 herbarium samples from four distantly related groups of flowering plants were used for DNA extraction and subsequent measurements of DNA purity and concentration. We did not find any significant relation between DNA purity and the age of the sample. However, DNA yields were different between plant groups studied. We believe that there there should be no reservations about "old" samples if the goal is to extract more DNA of better purity. We argue that the older herbarium samples are the mine for the future DNA studies, and have the value not less than the "fresh" specimens.
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http://dx.doi.org/10.7717/peerj.1087 | DOI Listing |
Sci Rep
January 2025
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt.
Alzheimer's disease (AD) poses a global health challenge, demanding innovative approaches for effective treatments. Clerodendrum infortunatum Linn. (Lamiaceae) is a shrub traditionally used as a medicinal plant to treat inflammation, skin diseases, and bronchitis.
View Article and Find Full Text PDFOecologia
January 2025
Plant Evolutionary Ecology, Institute of Ecology, Evolution and Diversity, Faculty of Biological Sciences, Goethe University Frankfurt, Max-Von-Laue-Str. 13, 60438, Frankfurt am Main, Germany.
Rapid environmental changes across Europe include warmer and increasingly variable temperatures, changes in soil nutrient availability, and pollinator decline. These abiotic and biotic changes can affect natural plant populations and force them to optimize resource use against competitors. To date, the evolution of competitive ability in the context of changes in nutrient availability remains understudied.
View Article and Find Full Text PDFNat Commun
December 2024
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland, 4072, Australia.
The relationship between intra-specific and inter-specific patterns and processes over evolutionary time is key to ecological investigations. We examine this relationship taking an approach of focussing on the association between vegetation and floristic classifications, summaries of inter-specific processes, and intra-specific genetic structuring. Applying an innovative, multispecies, and standardised population genomic approach, we test the relationship between vegetation mapping schemes and structuring of genetic variation across a large, environmentally heterogenous region in eastern Australia.
View Article and Find Full Text PDFMicrosc Res Tech
December 2024
Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo, Nigeria.
Croton lobatus L. and Croton zambesicus Mull. Arg.
View Article and Find Full Text PDFBMC Plant Biol
December 2024
Department of Pharmacognosy, Department of Pharmacy, Guilin Medical University, Guilin, 541199, China.
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