A protocol is presented for offline microfluidic NMR analysis hyphenated with supercritical chromatographic separation. The method demonstrates quantitative detection with good sensitivity. Typical sample amounts of 10 nanomoles can be detected in a fast and cost-effective manner.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c5an00772k | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
1-Octanol-assisted ultra-small volume droplet microfluidics with nanoelectrospray ionization mass spectrometry.
Anal Chim Acta
September 2024
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA. Electronic address:
Background: Droplet microfluidics with push-pull and microdialysis sampling from brain slices, cultured cells and engineered tissues produce low volume mass limited samples containing analytes sampled from the extracellular space. This sampling approach coupled to mass spectrometry (MS) detection allows evaluation of time-dependent chemical changes. Our goal is an approach for continuous sampling and segregation of extracellular samples into picoliter droplets followed by the characterization of the droplets using nanoelectrospray ionization (nESI) MS.
View Article and Find Full Text PDFHyperpolarized Nano-NMR Platform for Quantification of Mass Limited Samples.
Anal Chem
July 2024
Department of Radiology, Memorial Sloan Kettering Cancer Center, New York City, New York 10065, United States.
Metabolic flux analysis of live cells using NMR enables the study of cancer metabolism and response to treatment. However, conventional NMR platforms require often prohibitively high numbers of cells to achieve significant resolution. In this work, we present a double H/C resonance NMR probe consisting of a solenoid coil with a less than 100 nL sensitive region.
View Article and Find Full Text PDFAn Optimized Miniaturized Filter-Aided Sample Preparation Method for Sensitive Cross-Linking Mass Spectrometry Analysis of Microscale Samples.
Anal Chem
July 2024
Institute of Drug Discovery Technology, Ningbo University, Ningbo, Zhejiang 315211, China.
Article Synopsis
- Cross-linking mass spectrometry (XL-MS) is an important technique for studying protein structures and their interactions, but analyzing low-abundance samples can result in sample loss during preparation.
- The study introduces an optimized method called O-MICROFASP that enhances XL-MS analysis for tiny samples, significantly increasing the identification of cross-linked peptides from minimal amounts of protein.
- By using various cross-linkers and an advanced sample preparation technique, researchers identified over 2700 unique cross-linked peptides from just 5 μg of HeLa cell lysates, confirming the method's effectiveness for detailed protein analysis and interaction mapping.
Top-Down Proteomics Analysis of Picogram-Level Complex Samples Using Spray-Capillary-Based Capillary Electrophoresis-Mass Spectrometry.
Anal Chem
May 2024
Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, Oklahoma 73019, United States.
Proteomics analysis of mass-limited samples has become increasingly important for understanding biological systems in physiologically relevant contexts such as patient samples, multicellular organoids, spheroids, and single cells. However, relatively low sensitivity in top-down proteomics methods makes their application to mass-limited samples challenging. Capillary electrophoresis (CE) has emerged as an ideal separation method for mass-limited samples due to its high separation resolution, ultralow detection limit, and minimal sample volume requirements.
View Article and Find Full Text PDFSolid-Phase Microextraction-Aided Capillary Zone Electrophoresis-Mass Spectrometry: Toward Bottom-Up Proteomics of Single Human Cells.
J Am Soc Mass Spectrom
June 2024
Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, Michigan 48824, United States.
Capillary zone electrophoresis-mass spectrometry (CZE-MS) has been recognized as a valuable technique for the proteomics of mass-limited biological samples (i.e., single cells).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!