We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519776 | PMC |
| http://dx.doi.org/10.1038/srep12490 | DOI Listing |
Publication Analysis
Top Keywords
mononuclear cells
8
rapid column-free
4
column-free enrichment
4
enrichment mononuclear
4
cells solid
4
solid tissues
4
tissues developed
4
developed rapid
4
rapid negative
4
negative selection
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!