A single, simple, selective and validated high performance thin layer chromatographic (HPTLC) method was developed for the determination of either linagliptin (LGP), saxagliptin (SGP) or vildagliptin (VGP) in their binary mixtures with metformin (MET) in pharmaceutical preparations using environmentally preferable green mobile phase system. Separation was carried out on Merck HPTLC aluminum sheets of silica gel 60 F254 using methanol-0.5% w/v aqueous ammonium sulfate (8 : 2, v/v) as mobile phase. Densitometric measurement of the spots was performed at 225 nm for LGP/MET mixture and at 208 nm for both SGP/MET and VGP/MET mixtures. The linear regression analysis data were used for the regression line in the range of 0.05-0.5 µg/band for LGP and SGP and 0.2-2 and 5-40 µg/band for VGP and MET, respectively. The method was validated and showed good performances in terms of linearity, limits of detection and quantitation, precision, accuracy, selectivity and specificity. The calculated percentage relative error values and percentage relative standard deviation for intra- and interday precision studies did not exceed 2%. The developed method was satisfactorily applied for the analysis of pharmaceutical preparations and proved to be specific and accurate for the quality control of the cited drugs in their dosage forms.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/chromsci/bmv106 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Semi-field studies on biochemical markers of honey bee workers (Apis mellifera) after exposure to pesticides and their mixtures.
PLoS One
January 2025
Department of Bees Breeding, Institute of Animal Husbandry and Breeding, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
Article Synopsis
- Many pesticides used in agriculture can accumulate in the environment, exposing bees to multiple substances simultaneously, which is not commonly studied in research.
- The study focused on the chronic effects of pesticide mixtures on honey bee worker's hemolymph, using concentrations found in their natural environment.
- Results showed that acetamiprid decreased urea levels significantly, glyphosate had little effect, and tebuconazole, despite being considered safe, caused notable changes in several biochemical markers, indicating a need for further research on fungicides' impact on bees.
Microscopic Heterogeneity Driven by Molecular Aggregation and Water Dynamics in Aqueous Osmolyte Solutions.
J Chem Inf Model
January 2025
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of Korea.
Water dynamics are investigated in binary osmolyte-water mixtures, exhibiting a microscopic heterogeneity driven by molecular aggregation, on the basis of molecular dynamics (MD) simulation studies. The protecting osmolyte TMAO molecules in solution are evenly dispersed without the formation of noticeable osmolyte aggregates, while the denaturant TMU molecules aggregate readily, generating microscopic heterogeneity in the spatial distribution of component molecules in TMU-water mixtures. A combined study of MD simulation with graph theoretical analysis and spatial inhomogeneity measurement with -values in the two osmolyte solutions revealed that the translational and rotational motions of water in the microheterogeneous environment of TMU-water mixtures are less hindered than those in the homogeneous media of TMAO-water mixtures.
View Article and Find Full Text PDFFabricating pine needles derived spherical nanocellulose with polyaniline and montmorillonite clay for simultaneous removal of cationic and anionic dyes from binary mixtures.
Int J Biol Macromol
January 2025
Department of Chemistry, Himachal Pradesh University, Summerhill, Shimla, Himachal Pradesh 171005, India.
Herein, pine needles derived spherical nanocellulose (SNC) was combined with aniline to form SNC-polyaniline (SNC-PANI), followed by modification with montmorillonite (MMT) to form SNC-PANI-MMT composite. The as-synthesized materials were characterized by FTIR, XRD, XPS, TGA, FESEM, and EDS and evaluated for the simultaneous adsorption of cationic and anionic dyes, malachite green (MG), and Congo red (CR) from MG-CR mixture, and fuchsin basic (FB) and methyl orange (MO) from FB-MO mixture. Non-linear kinetics of adsorption showed the anionic dyes, CR and MO to follow pseudo-first order kinetics with 91.
View Article and Find Full Text PDFGeneralized functional varying-index coefficient model for dynamic synergistic gene-environment interactions with binary longitudinal traits.
PLoS One
January 2025
Department of Statistics and Probability, Michigan State University, East Lansing, MI, United States of America.
The genetic basis of complex traits involves the function of many genes with small effects as well as complex gene-gene and gene-environment interactions. As one of the major players in complex diseases, the role of gene-environment interactions has been increasingly recognized. Motivated by epidemiology studies to evaluate the joint effect of environmental mixtures, we developed a functional varying-index coefficient model (FVICM) to assess the combined effect of environmental mixtures and their interactions with genes, under a longitudinal design with quantitative traits.
View Article and Find Full Text PDFChanges in flavor profile of sauce-flavor baijiu: Perceptual interactions between 1-propanol and aroma compounds.
Food Chem X
January 2025
School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
1-Propanol is an important aroma compound in sauce-flavor Baijiu. However, the mechanism by which it affects the aroma of sauce-flavor Baijiu has not been fully investigated. In this study, an instrumental and perceptual analysis was employed to assess the impact of 1-propanol on the flavor and volatile compounds in sauce-flavor Baijiu.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!