Octreotide protects ovary against ischemia-reperfusion injury in rats: Evaluation of histological and biochemical parameters.

Aim: This study investigated the efficacy of octreotide for prevention of ischemia-reperfusion injury in rat ovary.

Methods: Thirty-two adult female rats were included. Rats were divided into five groups: in the sham group, the abdominal wall was only opened and closed; in the torsion group, ischemia was induced for 3 h using a torsion model involving atraumatic vascular clips; in the torsion/octreotide group, rats were given 100 µg/kg i.p. octreotide 30 min before torsion was induced; in the torsion/detorsion group, rats underwent 3 h ischemia-3 h reperfusion; in the torsion/detorsion/octreotide group, rats underwent 3 h ischemia followed by 100 µg/kg octreotide i.p. 30 min prior to 3 h reperfusion. Ovarian tissue damage was scored on histopathology. Ovarian tissue malondialdehyde and plasma pentraxin 3 were measured biochemically.

Results: In comparison with the sham group, both the torsion and torsion/detorsion groups had significantly higher scores for follicular degeneration, vascular congestion, edema, hemorrhage and leukocyte infiltration. Octreotide significantly decreased these scores in both groups. Ovarian malondialdehyde and plasma pentraxin 3 were significantly higher both in the torsion and torsion/detorsion groups compared with the sham group. Octreotide also decreased these levels significantly both in the torsion/octreotide and torsion/detorsion/octreotide groups.

Conclusion: Octreotide ameliorated the potential side-effects of ovarian ischemia-reperfusion injury in a rat model.