Introgressive hybridization of common wheat is still one of the most effective way of wheat genome enrichment with resistance genes to stresses. In this context, T genome of Amblyopyrum muticum is a promising one for such wheat improvement. Genome substitution amphidiploid Aurotica (AABBTT) with A and B subgenomes from common wheat cultivar Aurora and with T subgenome from Amblyopyrum muticum is characterized by high level of cold resistance. In wheat, this characteristic associated with chromosomes of the homoeologous group 5. Comparative microsatellite analysis of Aurora and Aurotica identified 9 SSR loci specific to D genome which produced other amplicons with Aurotica DNA spectrum. Primers for these loci were used for screening (Aurora x Aurotica)F5 progeny (234 plants) in purpose to find those individuals, which carry 5T chromosome or its fragments in their genomes. In this study were detected 24 plants with disomic substitutions 5D/5T. All of them were offspring of five F4 plants, which in turn derived from two F2 individuals. A significant number of plants contain recombinant chromosome 5D/5T. The recombination event could occur in any generation since F2.
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