Twenty-six wild-type Streptomyces strains tested for resistance to arsenate, arsenite and antimony(III) could be divided into four groups: those resistant only to arsenite (3) or to arsenate (2) and those resistant (8) or sensitive (13) to both heavy metals. All strains were sensitive to antimony. The structural genes for the ars operon of Escherichia coli were subcloned into various Streptomyces plasmid vectors. The expression of the whole ars operon in streptomycetes may be strain-specific and occurred only from low-copy-number plasmids. The arsC gene product could be expressed from high-copy plasmids and conferred arsenate resistance to both E. coli and Streptomyces species. The ars operon expressed in S. lividans and the arsC gene expressed in S. noursei did not render the synthesis of undecylprodigiosin and nourseothricin, respectively, phosphate-resistant. In addition in wild-type strains of Streptomyces phosphate sensitivity of antibiotic biosynthesis did not show strong correlation with resistance of growth to arsenicals.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/00221287-135-3-583 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome-wide analyses provide insights into genetic variation, phylo- and co-phylogenetic relationships, and biogeography of the entomopathogenic nematode genus Heterorhabditis.
Mol Phylogenet Evol
January 2025
Laboratory of Diagnosis and Integrated Management of Plant Bio-Aggressors. University of Parakou, BP123 Parakou, Borgou, Benin.
Multigene, genus-wide phylogenetic studies have uncovered the limited taxonomic resolution power of commonly used gene markers, particularly of rRNA genes, to discriminate closely related species of the nematode genus Heterorhabditis. In addition, conflicting tree topologies are often obtained using the different gene markers, which limits our understanding of the phylo- and co-phylogenetic relationships and biogeography of the entomopathogenic nematode genus Heterorhabditis. Here we carried out phylogenomic reconstructions using whole nuclear and mitochondrial genomes, and whole ribosomal operon sequences, as well as multiple phylogenetic reconstructions using various single nuclear and mitochondrial genes.
View Article and Find Full Text PDFPavlovian-Type Learning in Environmental Bacteria: Regulation of Herbicide Resistance by Arsenic in Pseudomonas putida.
Environ Microbiol
December 2024
Systems Biology Department, Centro Nacional de Biotecnología-CSIC, Madrid, Spain.
The canonical arsRBC genes of the ars1 operon in Pseudomonas putida KT2440, which confer tolerance to arsenate and arsenite, are followed by a series of additional ORFs culminating in phoN1. The phoN1 gene encodes an acetyltransferase that imparts resistance to the glutamine synthetase inhibitor herbicide phosphinothricin (PPT). The co-expression of phoN1 and ars genes in response to environmental arsenic, along with the physiological effects, was analysed through transcriptomics of cells exposed to the oxyanion and phenotypic characterization of P.
View Article and Find Full Text PDFMechanism of antimony oxidation and adsorption using immobilized Klebsiella aerogenes HC10 in soil.
Sci Total Environ
December 2024
School of Resources, Environment and Materials, Guangxi University, Nanning 530004, China. Electronic address:
Klebsiella aerogenes HC10 is one of the few strains isolated from contaminated soil that efficiently oxidizes Sb. However, the sensitivity of microorganisms to environmental conditions limits Sb-oxidizing bacteria applications in soil remediation. Immobilizing Sb-oxidizing bacteria is a promising strategy to improve colonization rates and microorganism inefficiencies and to strengthen bioremediation in Sb-contaminated soil.
View Article and Find Full Text PDFUnravelling the mechanism of arsenic resistance and bioremediation in Stenotrophomonas maltophilia: A molecular approach.
Environ Pollut
December 2024
Department of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai- 400005, Maharashtra, India. Electronic address:
Article Synopsis
- The study investigates how the bacterium S. maltophilia resists and detoxifies arsenic, despite the overall limited understanding in this area.
- Advanced analytical techniques revealed that S. maltophilia shows minimal changes on its cell surface when exposed to arsenic, but it does accumulate arsenic internally.
- The research found that S. maltophilia activates various protective enzymes and possesses specific genes that facilitate arsenic reduction and detoxification, highlighting a complex molecular mechanism for bioremediation.
First Report of a Phytoplasma Strain in the Elm Yellows Group (16SrV) associated with Virginia Creeper in Maryland, USA.
Plant Dis
August 2024
USDA-APHIS-PPQ, CPHST-Beltsville Laboratory , BARC-East, Bldg-580, Powder Mill Road, Beltsville, Maryland, United States, 20705-2350;
Article Synopsis
- Virginia creeper is a fast-growing deciduous vine native to eastern North America, often used for ornamental purposes due to its attractive fall foliage.
- In July 2022, two Virginia creeper vines in Lanham, MD, showed signs of yellow mottling and premature reddening of leaves, prompting an investigation that involved collecting leaf samples for DNA analysis.
- Results from a specific PCR test confirmed the presence of a phytoplasma, leading to the identification of a new strain named VC-MD1; this strain is closely related to previously known phytoplasmas that affect other plants, demonstrating a potential link to broader plant health issues.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!