Vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. In this study, we developed a cross-priming amplification (CPA) assay coupled with vertical flow (VF) visualization for rapid and sensitive detection of V. parahaemolyticus. This assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). Small concentrations of V. parahaemolyticus (1.8 CFU/mL for pure cultures and 18 CFU/g for reconstituted samples) were detected within 1 h. CPA-VF can be applied at a large scale and can be used to detect V. parahaemolyticus strains rapidly in seafood and environmental samples, being especially useful in the field.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mcp.2015.07.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel cross-priming amplification technique combined with lateral flow strips for rapid and visual detection of zoonotic Toxoplasma gondii.
Vet Parasitol
January 2025
Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, PR China. Electronic address:
Toxoplasma gondii, an obligate intracellular protozoan, infects almost all warm-blooded animals and humans, with felines serving as its sole definitive hosts. Cats release T. gondii oocysts into the environment through feces, contributing to environmental contamination that can lead to toxoplasmosis in humans upon exposure through ingestion of contaminated food, water, or soil.
View Article and Find Full Text PDFRapid Visual Detection of Red Sea Bream Iridovirus Using a Novel Cross-Priming Amplification-Based Lateral Flow Assay.
J Fish Dis
December 2024
Department of Aquatic Life Medicine, Pukyong National University, Busan, Republic of Korea.
Red sea bream iridovirus (RSIV) occurs mainly at high water temperatures and infects more than 30 different species of fish. In Asia, infected fish cause mass mortality every year. Molecular diagnostics is a technology that efficiently detects and identifies a wide range of fish pathogens through rapid and sensitive analysis of their genetic material.
View Article and Find Full Text PDF[Clinical value of automated EasyNAT system for the diagnosis of tuberculosis in paraffin-embedded tissues].
Beijing Da Xue Xue Bao Yi Xue Ban
December 2024
Department of Pathology, Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China.
Objective: Assessing the accuracy of automated EasyNAT system for rapidly detecting paraffin-embedded tissue for the diagnosis of tuberculosis.
Methods: A retrospective analysis was conducted on 134 patients, comprising 101 with confirmed tuberculosis and 33 without tuberculosis, treated at Beijing Chest Hospital, Capital Medical University, between 2018 and 2022.The clinical diagnostic results served as the standard for assessing the diagnostic performance of the EasyNAT system in comparison to quantitative real-time polymerase chain reaction (qPCR) for tuberculosis detection in paraffin-embedded tissues.
Diagnostic Value of Cross-priming Amplification Combined With CRISPR-Cas12b in Detecting Cell-free DNA in Tuberculous Pleural Effusion.
Open Forum Infect Dis
December 2024
Clinical Laboratory Center, Hangzhou Red Cross Hospital, Hangzhou, China.
Background: Diagnosis of tuberculous pleural effusion (TPE) remains challenging. Studies have shown that detecting cell-free (cf-TB) DNA in pleural effusion can improve TPE diagnosis. This study aimed to evaluate the diagnostic value of our recently developed TB One-Pot assay, which combines cross-priming amplification with CRISPR-Cas12b, in detecting cf-TB for TPE.
View Article and Find Full Text PDFEstablishment of cross-priming amplification for point-of-care detection of and non-tuberculosis mycobacteria.
Heliyon
October 2024
Department of Clinical Laboratory, Longyan First Affiliated Hospital of Fujian Medical University, Longyan, Fujian, China.
Article Synopsis
- - The study aims to simplify sputum sample preparation for point-of-care testing (POCT) of MTB and non-tuberculosis mycobacteria (NTM) using Cross-Priming Amplification (CPA) technology, particularly in grassroots settings in China.
- - High-concentration guanidine thiocyanate (GTC) was found effective in liquefying sputum and inactivating MTB, with the new CPA method showing strong agreement with traditional RT-PCR testing results.
- - The CPA method demonstrates good accuracy in distinguishing between MTB and NTM, suggesting it is a rapid, simple, and effective alternative for testing, eliminating the need for specialized laboratory facilities.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!