AI Article Synopsis
- Approximately 50 genes, beyond heat shock proteins, show expression changes under thermal stress, with nitric oxide synthase (NOS) genes being particularly significant for adaptation in animals.
- The study focused on examining the expressions of various NOS types and heat shock proteins (HSP70 and HSP90) in Barbari goats across different seasons using techniques like real-time PCR and western blotting.
- Findings revealed that NOS and HSP expressions were significantly higher during extreme temperatures (peak summer and winter), suggesting these genes play a crucial role in helping goats cope with thermal stress and maintain cellular stability.
Article Abstract
Approximately 50 genes other than heat shock protein (HSP) expression changes during thermal stress. These genes like nitric oxide synthase (NOS) need proper attention and investigation to find out their possible role in the adaptation to thermal stress in animals. So, the present study was undertaken to demonstrate the expressions of inducible form type II NOS (iNOS), endothelial type III NOS (eNOS), constitutively expressed enzyme NOS (cNOS), HSP70, and HSP90 in peripheral blood mononuclear cells (PBMCs) during different seasons in Barbari goats. Real-time polymerase chain reaction, western blot, and immunocytochemistry were applied to investigate messenger RNA (mRNA) expression, protein expression, and immunolocalization of examined factors. The mRNA and protein expressions of iNOS, eNOS, cNOS, HSP70, and HSP90 were significantly higher (P < 0.05) during peak summer, and iNOS and eNOS expressions were also observed to be significantly higher (P < 0.05) during peak winter season as compared with moderate season. The iNOS, eNOS, cNOS, HSP70, and HSP90 were mainly localized in plasma membrane and cytoplasm of PBMCs. To conclude, data generated in the present study indicate the possible involvement of the NOS family genes in amelioration of thermal stress so as to maintain cellular integrity and homeostasis in goats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00484-015-1035-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Accurate assessment of the three-dimensional shrinkage stress evolution for photopolymerized dental filling materials: Mechano-chemo-thermo-coupled finite element modeling and experimental validation.
Dent Mater
January 2025
Department of Engineering Mechanics, School of Civil Engineering, Wuhan University, Wuhan, Hubei 430072, China; Wuhan University Shenzhen Research Institute, Shenzhen 518108, China. Electronic address:
Objective: Photopolymerized resin composites are widely used as dental filling materials. However, the shrinkage stress generated during photopolymerization can lead to marginal microcracks and eventual restoration failure. Accurate assessment of the stress evolution in dental restorations, particularly in complex cavity geometries, is critical for improving the performance and longevity of the dental filling materials.
View Article and Find Full Text PDFComparative analysis of Modified Johnson-Cook model and Artificial Neural Network for flow stress prediction in BN-reinforced AZ80 magnesium composite.
J Phys Condens Matter
January 2025
University of Science and Technology Beijing, No. 30, Xueyuan Road, Haidian District, Beijing, 100083, CHINA.
Boron nitride (BN), renowned for its exceptional optoelectrical properties, mechanical robustness, and thermal stability, has emerged as a promising two-dimensional (2D) material. Reinforcing AZ80 magnesium alloy with BN can significantly enhance its mechanical properties. To investigate and predict this enhancement during hot deformation, we introduce two independent modeling approaches a modified Johnson-Cook (J-C) constitutive model and an Artificial Neural Network (ANN).
View Article and Find Full Text PDFIntermittent Thermal Convection in Jammed Emulsions.
Phys Rev Lett
December 2024
Department of Physics and INFN, Tor Vergata University of Rome, Via della Ricerca Scientifica 1, 00133 Rome, Italy.
We study the process of thermal convection in jammed emulsions with a yield-stress rheology. We find that heat transfer occurs via an intermittent mechanism, whereby intense short-lived convective "heat bursts" are spaced out by long-lasting conductive periods. This behavior is the result of a sequence of fluidization-rigidity transitions, rooted in a nontrivial interplay between emulsion yield-stress rheology and plastic activity, which we characterize via a statistical analysis of the dynamics at the droplet scale.
View Article and Find Full Text PDFMechanisms of thermal, acid, desiccation and osmotic tolerance of spp.
Crit Rev Food Sci Nutr
January 2025
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
spp. exhibit remarkable resilience to extreme environmental stresses, including thermal, acidic, desiccation, and osmotic conditions, posing significant challenges to food safety. Their thermotolerance relies on heat shock proteins (HSPs), thermotolerance genomic islands, enhanced DNA repair mechanisms, and metabolic adjustments, ensuring survival under high-temperature conditions.
View Article and Find Full Text PDFFailure risk study of anchor bolts durability for loess slope under long-term hydro-thermal effect.
Sci Rep
January 2025
School of Highway, Chang'an University, Xi'an, 710064, Shaanxi, China.
The long-term safety and durability of anchor systems are the focus of slope maintenance management and sustainable operation. This study presents the observed temperature, humidity, and anchor bolt stress at varying depths from four-year remote real-time monitoring of the selected loess highway cut-slope. The potential correlation between slope hydrothermal environment and anchor stress is analyzed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!