We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675668 | PMC |
| http://dx.doi.org/10.1021/acs.nanolett.5b01047 | DOI Listing |
Publication Analysis
Top Keywords
cancer cells
8
energy transfer
8
activatable cell-penetrable
4
cell-penetrable multiplex
4
fret
4
multiplex fret
4
fret nanosensor
4
nanosensor profiling
4
profiling mt1-mmp
4
mt1-mmp activity
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!