Monitoring the formation of cholesterol oxidation products in model systems using response surface methodology.

Lipids Health Dis

Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, 51-921, Korea.

Published: July 2015

AI Article Synopsis

  • Cholesterol oxidation products (COPs) are harmful compounds formed during the cooking of animal foods and their formation can be influenced by factors like pH, fatty acids, and temperature.
  • The study found that higher pH levels and shorter cooking times significantly reduced COP formation, while unsaturated fatty acids increased it under low-temperature conditions.
  • Cooking at temperatures above 200 °C initially raised COP levels, but these levels decreased with longer heating times. The major COPs identified were α-epoxide, 20α-hydroxycholesterol, and 25-hydroxycholesterol.

Article Abstract

Background: Cholesterol oxidation products (COPs) are produced during thermal processing of animal origin foods and are considered to have negative health impacts. The model systems are helpful to understand the impact of various factors on oxidation changes in foods during cooking process.

Methods: The study presented herein investigates the effects of pH, presence of unsaturated fatty acids, and heat on the formation of cholesterol oxidation products (COPs). Two model systems were designed to investigate the formation of cholesterol oxidation products in different lipid environments. The cholesterol oxides produced were quantified using gas chromatography.

Results: The level of cholesterol oxidation products decreased significantly at higher pH (above 5.8) and shorter heating time (3 h). The presence of unsaturated fatty acids (linoleic and oleic acids) significantly increased the amount of COPs under low-temperature heating conditions (100 °C and 1 h) but did not affect the production of COPs at higher temperature (150 °C). Increasing the temperature to 200 °C significantly increased the amount of COPs during the first hour of heating and this amount decreased upon further heating. The most frequently observed COPs in samples were α-epoxide, 20α-hydroxycholesterol, and 25-hydroxycholesterol.

Conclusions: In conclusion, pH below 5.8, presence of unsaturated fatty acid, and high cooking temperature (>150 °C) leads to increased production of cholesterol oxidation products.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512089PMC
http://dx.doi.org/10.1186/s12944-015-0074-6DOI Listing

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