AI Article Synopsis
- Arabidopsis mutants created using CRISPR/Cas9 often exhibit mosaicism in the T1 generation, meaning not all cells have the same edits.
- In this study, researchers employed egg cell-specific promoters to achieve high-efficiency, non-mosaic T1 mutants for multiple target genes.
- They found that the success of this gene-editing method was influenced by the choice of terminator and that combining two egg cell-specific promoters significantly increased mutation efficiency compared to using single promoters.
Article Abstract
Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two egg cell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507317 | PMC |
| http://dx.doi.org/10.1186/s13059-015-0715-0 | DOI Listing |
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