Calibration of the fructosamine assay using albumin solutions glycated in vitro.

Ann Clin Biochem

Clinical Chemistry Department, Warwick Hospital, UK.

Published: November 1989

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http://dx.doi.org/10.1177/000456328902600618DOI Listing

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Albumin extracted from serum by a simple technique using trichloroacetic acid and ethanol has been applied to a fructosamine assay using nitroblue tetrazolium. A fructosamine assay using extracted albumin sample was carried out without interference from low molecular weight substances with reducing activities and other proteins with varying concentrations, half-lives and reducing activities. 1-Deoxy-1-p-toluidino-D-fructose (DTF) was selected as a calibrator for the assay because it is a stable Amadori reaction product with a constant glycation rate.

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A major goal in the treatment of type 2 diabetes is to maintain blood glucose values in the normal or near normal range. A system of monitoring glycemic control, which includes self-monitoring of blood glucose (SMBG) by the patient, and frequent glycated protein assays, is an important tool for achieving this goal. Optimal use of SMBG by the patient is best accomplished through comprehensive glucose monitoring education, which includes appropriate monitor selection and use and strategies to use monitoring results to improve glycemic control.

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