Aim: The molecular pathways regulating cartilage degradation are unclear. miR-381 was identified as a putative regulator of chondrogenesis related genes. Here, we examined its role in chondrogenesis and osteoarthritic cartilage degeneration.
Methods: miR-381 expression was assessed in vitro in response to IL-1β stimulation in primary human (PHC) and mouse (PMC) chondrocytes, and ATDC5 derived chondrocytes; and in vivo in mouse embryos and human osteoarthritic cartilage. The effects of miR-381 on chondrogenesis and NF-kB signaling were assessed using a synthetic RNA mimic or inhibitor and luciferase assay, respectively. Upstream regulators of miR381 were probed using siRNA or overexpression plasmids for Sox9 and Runx2.
Results: miR-381 expression was elevated in chondrogenic and hypertrophic ATDC5 cells. miR-381 was induced in vitro by IL-1β in ATDC5 cells, PMCs, and PHCs, and was expressed in areas of cartilage degradation or absorption in vivo. Overexpression of Runx2 or Sox9 increased miR-381 expression in ATDC5 cells. miR-381 suppressed expression of collagen, type II, alpha 1, and enhanced expression of metalloproteinase-13 (MMP-13), but did not regulate NFKBIA and NKRF activity.
Conclusion: miR-381 was highly expressed during chondrogenesis and in arthritic cartilage. It may contribute to absorption of the cartilage matrix by repressing type II collagen and inducing MMP-13.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1159/000430148 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Transcriptional profiling of exosomes derived from serum of patients with rare earth pneumoconiosis by RNA-sequencing and PI3K/Akt pathway is activated in lung of mice exposed to rare earth NdO.
Toxicol Lett
January 2025
Department of Public Health,International College,Krirk University, Bangkok 10220, Thailand; School of Public Health, Baotou Medical College, Baotou 014030, Inner Mongolia, PR China. Electronic address:
Rare earth is used extensively around the world, and rare earth particles cause a respiratory disease in workers termed rare earth pneumoconiosis(REP) that have attracted considerable attention. However, the mechanisms of REP, characterized by diffuse pulmonary fibrosis, are elusive. REP progression involves various signaling pathway networks comprising numerous cell types and cytokines.
View Article and Find Full Text PDF[Screening and identification of key miRNAs in post-transcriptional regulation of CART in the bovine hypothalamus].
Sheng Wu Gong Cheng Xue Bao
December 2024
College of Animal Science, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
This study aimed to explore the roles of microRNAs (miRNAs) in the post-transcriptional regulation of cocaine- and amphetamine-regulated transcript (CART) peptide in the bovine hypothalamus and to screen key regulatory miRNAs. Targetscan was used to predict the potential miRNAs binding to 3' untranslated regions (3'UTR). Bioinformatics analysis predicted 7 miRNA binding sites in the bovine 3'UTR, which were bta-miR-377, bta-miR-331-3p, bta-miR-491, bta-miR-493, bta-miR-758, bta-miR-877, and bta-miR-381, respectively.
View Article and Find Full Text PDFExploring genetic signatures of obesity: hub genes and miRNAs unveiled through comprehensive bioinformatic analysis.
J Diabetes Metab Disord
December 2024
Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Objectives: Adipogenesis, the process of fat accumulation in adipose tissue, is closely linked to obesity, a condition characterized by excessive fat storage. Genetic factors significantly contribute to an individual's susceptibility to adipogenesis and the development of obesity.
Methods: In this study, we conducted a comprehensive bioinformatic analysis, including Weighted Gene Co-expression Analysis, differentially expressed gene analysis, and protein-protein interaction analysis, to identify hub genes and miRNAs associated with obesity.
EIF4A3-mediated oncogenic circRNA hsa_circ_0001165 advances esophageal squamous cell carcinoma progression through the miR-381-3p/TNS3 pathway.
Cell Biol Toxicol
October 2024
Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Article Synopsis
- Esophageal squamous cell carcinoma (ESCC) has a poor prognosis and lacks effective treatment targets, making it a significant clinical challenge.
- A study identified that the circular RNA hsa_circ_0001165 is overexpressed in ESCC tissues and cell lines, correlating with more advanced disease stages, and its knockdown reduces cancer cell aggressiveness and tumor growth.
- Hsa_circ_0001165 enhances the expression of tensin-3 (TNS3) by sponging the miRNA miR-381-3p, indicating its potential as a biomarker and therapeutic target for innovative ESCC treatment strategies.
Bone marrow mesenchymal stem cells-derived exosomal miR-381 alleviates lung ischemia-reperfusion injury by activating Treg differentiation through inhibiting YTHDF1 expression.
Cell Signal
December 2024
Department of Thoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu, China. Electronic address:
Aim: Our study aimed to investigate whether BMSCs-derived exosomal miR-381 promotes Treg cell differentiation in lung ischemia-reperfusion injury (LIRI), and the underlying mechanism.
Methods: The in vitro and in vivo models of LIRI were established by hypoxia/reoxygenation (H/R) treatment and lung ischemia/reperfusion (I/R) surgery, respectively. BMSCs-derived exosomes were isolated and identified by western blot, nanoparticle tracking analysis, and transmission electron microscopy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!