AI Article Synopsis
- The study examined levels of various amino acids in different layers of rat retinas using microdissection and HPLC analysis.
- Glutamate and glutamine concentrations were found to be high in the inner nuclear and ganglion cell layers, while aspartate was more concentrated in the outer nuclear layer and outer plexiform layer.
- Glycine and GABA peaked in the inner plexiform layer, and taurine exhibited its highest levels in the outer nuclear layer.
Article Abstract
Concentrations of glutamate, aspartate, glutamine, glycine, GABA, and taurine were determined in samples microdissected from rat retinal layers and assayed by HPLC. Glutamate and glutamine were relatively high in the inner nuclear (INL) and ganglion cell (GCL) layers; aspartate was relatively high in the outer nuclear layer (ONL), outer plexiform layer, and INL. Distributions of glutamate and aspartate did not correlate well with those of enzymes involved in their metabolism. Glycine and GABA were highest in the inner plexiform layer, with increasing concentrations through the INL, and were relatively high in the GCL. Taurine was highest in the ONL.
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| http://dx.doi.org/10.1016/0042-6989(89)90055-2 | DOI Listing |
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