Emerging evidence has indicated that anandamide (AEA) is able to stimulate vasorelaxation in both spontaneously hypertensive rats (SHRs) and L-NAME-induced hypertensive rats. Yet it remains unknown whether AEA modulates vasomotion of the aorta in renovascular hypertensive (RVH) rats. The aim of present study is to explore the effect of AEA on the relaxation of thoracic aortas in two-kidney one-clip (2K1C)-induced RVH rats. It is demonstrated that AEA stimulates a pronounced relaxation in the aortas of 2K1C rats compared with sham rats. The enhanced relaxation caused by AEA in aortas from 2K1C rats was diminished in the presence of the cannabinoid receptor-1 (CB ) antagonist AM251 and the CB receptor antagonist AM630. Likewise, the vasodilation action of AEA was blocked in L-NAME-treated or endothelium-denuded aortas. The Western blot results revealed that the expression of CB and CB receptors was increased in the 2K1C rat aortas compared with sham rats. The phosphorylation of endothelial nitric oxide synthase (p-eNOS) at the activation site Ser1177 was enhanced in AEA-treated rings from 2K1C rats in both time-dependent and dose-dependent manners. The augmented p-eNOS expression was inhibited by the co-treatment with AM251 or AM630. Taken together, the present study demonstrated that AEA enhanced endothelium-dependent aortic relaxation through activation of both CB and CB receptors and P-eNOS/NO pathway in 2K1C rats.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/1440-1681.12450 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chronic cannabidiol treatment induces cardiovascular improvement in renovascular hypertensive rats.
J Hypertens
January 2025
Biotechnology Center, Federal University of Paraiba, João Pessoa, Brazil.
Article Synopsis
- Cannabidiol (CBD) shows promise in treating cardiovascular issues related to neurodegenerative diseases and has previously shown benefits in reducing blood pressure and oxidative stress in acute studies.
- This study aimed to evaluate the long-term effects of chronic CBD treatment specifically on rats with renovascular hypertension induced by a surgical model.
- Results indicated that chronic CBD significantly lowered blood pressure, improved vascular function, and reduced harmful reactive oxygen species, suggesting its potential as a therapeutic option for cardiovascular problems in hypertension.*
Mechanism Actions of Coniferyl Alcohol in Improving Cardiac Dysfunction in Renovascular Hypertension Studied by Experimental Verification and Network Pharmacology.
Int J Mol Sci
September 2024
Natural and Biomimetic Medicine Research Center, Tissue-Orientated Property of Chinese Medicine Key Laboratory of Sichuan Province, West China School of Medicine, West China Hospital, Sichuan University, Chengdu 610000, China.
Article Synopsis
- Renovascular hypertension (RH) is a type of secondary hypertension that can harm heart health, increasing the risk of cardiovascular diseases.
- The study explores the effects of Coniferol (CA), known for its ability to relax blood vessels, on RH-related heart conditions using a specific mouse model and heart cell line.
- Results show CA lowers blood pressure and heart inflammation, targeting specific inflammatory pathways, making it a potential therapy for RH-induced heart issues.
OXGR1-Dependent (Pro)Renin Receptor Upregulation in Collecting Ducts of the Clipped Kidney Contributes to Na Balance in Goldblatt Hypertensive Mice.
Int J Mol Sci
September 2024
Institute of Chemistry, Pontificia Universidad Catoólica de Valparaióso, Valparaióso 2340000, Chile.
Article Synopsis
- * The (pro)renin receptor (PRR), important for sodium reabsorption and blood pressure control, is upregulated in intercalated cells of the collecting duct in 2K1C mice, indicating a connection between reduced blood flow and increased blood pressure.
- * Experiments reveal that blocking OXGR1 or using OXGR1 knockout mice hampers PRR upregulation in response
Sarcoplasmic reticulum calcium ATPase (SERCA) proteolysis by matrix metalloproteinase-2 contributes to vascular dysfunction in early hypertension.
Eur J Pharmacol
November 2024
Department of Pharmacology, Ribeirao Preto Medical School, Brazil. Electronic address:
Aims: Hypertension is associated with an increased activity of matrix metalloproteinase (MMP)-2 in the vasculature, which, in turn, proteolyzes extra- and intracellular proteins that lead to vascular dysfunction. The activity of sarcoplasmic reticulum calcium ATPase (SERCA) is decreased in the aortas of hypertensive rats. Increased activity of MMP-2 proteolyzed SERCA in rat heart during ischemia and reperfusion injury, thus impairing cardiac function.
View Article and Find Full Text PDFCardiovascular and Renal Effects Induced by Alpha-Lipoic Acid Treatment in Two-Kidney-One-Clip Hypertensive Rats.
Biomedicines
August 2024
Laboratory of Nutrition, Physical Activity and Phenotypic Plasticity, Federal University of Pernambuco-UFPE, Vitória de Santo Antão 55608-680, Brazil.
α-Lipoic acid (LA) is an antioxidant of endogenous production, also obtained exogenously. Oxidative stress is closely associated with hypertension, which causes kidney injury and endothelial dysfunction. Here, we evaluated the cardiovascular and renal effects of LA in the two-kidney-one-clip (2K1C) hypertension model.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!