AI Article Synopsis
- The study investigates how microRNA (miRNA), specifically miR-155, influences gene expression and cellular responses by focusing on its interaction with a single target gene, SOCS1.
- Researchers created a mutation in SOCS1's 3' UTR to disrupt its regulation by miR-155 and examined the effects under normal and stressed conditions like autoimmune inflammation or viral infection.
- Findings revealed that while some immune functions dependent on miR-155 were directly linked to SOCS1, others were influenced by different mechanisms, suggesting the importance of context and cell type in miRNA regulation.
Article Abstract
MicroRNA (miRNA)-dependent regulation of gene expression confers robustness to cellular phenotypes and controls responses to extracellular stimuli. Although a single miRNA can regulate expression of hundreds of target genes, it is unclear whether any of its distinct biological functions can be due to the regulation of a single target. To explore in vivo the function of a single miRNA-mRNA interaction, we mutated the 3' UTR of a major miR-155 target (SOCS1) to specifically disrupt its regulation by miR-155. We found that under physiologic conditions and during autoimmune inflammation or viral infection, some immunological functions of miR-155 were fully or largely attributable to the regulation of SOCS1, whereas others could be accounted only partially or not at all by this interaction. Our data suggest that the role of a single miRNA-mRNA interaction is dependent on cell type and biological context.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4529747 | PMC |
| http://dx.doi.org/10.1016/j.immuni.2015.04.022 | DOI Listing |
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