Tobacco ringspot virus (TRSV, genus Nepovirus), causes severe diseases in soybean and tobacco plants. TRSV-induced bud blight disease significantly reduced both the yield and quality of soybeans. The function of the encoded viral gene product involved in TRSV infection was unclear due to the limitation of reverse genetics studies on the viral genome. Here, we represent the successful construction of infectious full-length cDNA clones of TRSV genome (RNA1 and RNA2). The cDNAs of TRSV RNA1 and RNA2 were cloned into the binary vector pPZP211 immediately downstream of a double cauliflower mosaic virus 35S promoter and upstream of the nopaline synthase terminator. Seven days after agrobacterium-mediated co-inoculation of these two constructs, Nicotiana benthamiana plants developed a systemic infection with necrotic ringspot symptoms and weak stunting of the leaves, similar to that induced by natural TRSV. The systemic infection was confirmed by transmission electron microscopy and Western blot analysis. Simultaneously, soybean, tomato, and Arabidopsis ecotype Estland were mechanically inoculated with sap prepared from TRSV-agroinfiltrated N. benthamiana leaves, showing typical symptoms of bud blight, necrotic spots, and lethal systemic necrosis, respectively. The system developed herein will be an appealing way to determine TRSV viral gene functions and study host-TRSV interactions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11262-015-1221-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
First Report of Diplodia Shoot Blight and Canker Disease Caused by on Ponderosa Pine in Colorado, USA.
Plant Dis
November 2024
Colorado State University, Department of Agricultural Biology, 1177 Campus Delivery, Fort Collins, Colorado, United States, 80523;
Article Synopsis
- Diplodia shoot blight and canker disease (DSB) is caused by the fungal pathogen Diplodia sapinea and primarily affects 2-3 needled pines, such as ponderosa pine, resulting in various symptoms including necrotic needles, cankers, and dieback.
- The pathogen can exist without visible symptoms in trees, making it difficult to detect, and outbreaks are more common in stressed environments like nurseries and seed orchards.
- Although D. sapinea has not been previously reported in Colorado, studies confirmed its presence and pathogenicity after observing symptoms in ponderosa pines in Wyoming in 2018 and discovering symptomatic trees in Colorado in 2021.
Competition for nutrient niches within the apple blossom microbiota antagonizes the initiation of fire blight infection.
New Phytol
September 2024
Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365, Korea.
Changes in the plant microbiota composition are intimately associated with the health of the plant, but factors controlling the microbial community in flowers are poorly understood. In this study, we used apple flowers and fire blight as a model system to investigate the effects of floral microbiota and microbial competition on disease development and suppression. To compare changes in microbial flora with the RNA expression patterns of plants, the flower samples were collected in three different flowering stages (Bud, Popcorn, and Full-bloom).
View Article and Find Full Text PDFFirst Report of Causing Black Scorch and Rot Disease on Date Palms in Arizona.
Plant Dis
April 2024
University of Arizona, Yuma Agriculture Center, 6425 W. 8th Street, Yuma, Arizona, United States, 85364;
Article Synopsis
- Phoenix dactylifera, commonly known as the date palm, is significant for its fruit production in the southwestern US, particularly in the Yuma area and Hyder Valley of Arizona, with around 4900 hectares commercially cultivated.
- In October 2022, date palms in the Phoenix Metropolitan area exhibited severe rot, starting with brown spots that advanced to black scorch symptoms, leading to leaf wilting and the collapse of the terminal bud.
- The disease was linked to a fast-growing fungus identified through DNA sequencing, revealing a high similarity to Neodeightonia phoenicum strains responsible for similar symptoms in other parts of the world.
Biological characteristics of the bacteriophage LDT325 and its potential application against the plant pathogen .
Front Microbiol
March 2024
College of Agriculture and Agricultural Engineering, Liaocheng University, Liaocheng, China.
Bud blight disease caused by is a major bacterial disease of tea plants in China. Concerns regarding the emergence of bacterial resistance to conventional copper controls have indicated the need to devise new methods of disease biocontrol. Phage-based biocontrol may be a sustainable approach to combat bacterial pathogens.
View Article and Find Full Text PDFFirst Report of Green Mould on Leaf of Caused by in Liaoning, China.
Plant Dis
February 2024
Microbial Research Institute of Liaoning Province, Chaoyang, Liaoning, China;
Article Synopsis
- - Phaseolus vulgaris, commonly known as common beans, was found to be affected by a green mold disease in greenhouses in Liaoning, China, from 2019 to 2022, showing symptoms like chlorotic lesions and a distinctive “V” shape spread.
- - Researchers collected 15 leaf samples with clear disease symptoms and isolated the fungi, which grew uniform colonies on potato dextrose agar; the characteristics of the isolated fungus indicated it was Cladosporium tenuissimum.
- - For further identification, the representative isolate KZ-19 underwent molecular analysis with specific genes sequenced, helping confirm the identity of the pathogen affecting the bean plants.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!