With increasing focus on sustainable energy, bio-refining from lignocellulosic biomass has become a thrust area of research. With most of the works being focused on biofuels, significant efforts are also being directed towards other value added products. Feruloyl esterases (EC. 3.1.1.73) can be used as a tool for bio-refining of lignocellulosic material for the recovery and purification of ferulic acid and related hydroxycinnamic acids ubiquitously found in the plant cell wall. More and more genes coding for feruloyl esterases have been mined out from various sources to allow efficient enzymatic release of ferulic acid and allied hydroxycinnamic acids (HCAs) from plant-based biomass. A sum up on enzymatic extraction of HCAs and its recovery from less explored agro residual by-products is still a missing link and this review brushes up the achieved landmarks so far in this direction and also covers a detailed patent search on this biomass refining enzyme.
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| http://dx.doi.org/10.1016/j.biortech.2015.06.117 | DOI Listing |
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Late-stage changes in the composition of cell walls of maize plants expressing an apoplast targeted, senescence enhanced fungal ferulic acid esterase, and the subsequent effects on tissue saccharification.
PLoS One
January 2025
Department of Biology, The Pennsylvania State University, University Park, PA, United States of America.
Article Synopsis
- The study explored the effects of a specific enzyme (FAEA) expressed in maize that targets the apoplast, focusing on its activity during the late stages of plant senescence and after storing the plant material.
- FAEA levels increased until the reproductive (R) stage but dropped during full leaf senescence (R+), while the enzyme remained stable even after six months of cold storage.
- The research found that FAEA expression led to decreased cell wall components like ferulates and improved the breakdown (saccharification) of plant material by enzymes, making it easier to extract sugars at later development stages.
Mining of latent feruloyl esterase resources in rumen and insight into dual-functional feruloyl esterase-xylanase from Pecoramyces ruminantium F1.
Bioresour Technol
November 2024
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, IL 61801, USA; Department of Animal Science, University of Illinois at Urbana-Champaign, IL 61801, USA; Department of Microbiology, University of Illinois at Urbana-Champaign, IL 61801, USA; Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, IL 61801, USA; Center for East Asian and Pacific Studies, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Feruloyl esterase (FAE) has been extensively studied for its crucial auxiliary effect in the biodegradation of lignocellulose. In this study, a FAE database including 15,293 amino acid sequences was established to gain a better understanding of rumen FAEs through multi-omics analysis. The higher expression level of rumen fungal FAEs over bacterial FAEs suggests that rumen fungi may have more important role in the lignocellulose degradation.
View Article and Find Full Text PDFCharacterization of heat- and alkali-resistant feruloyl esterase from Humicola insolens and application in the production of high-strength kraft straws.
Int J Biol Macromol
December 2024
School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China; State Key Laboratory of Bio-based Materials and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, Shandong, PR China. Electronic address:
Article Synopsis
- * Researchers expressed a thermostable alkaline form of FAE from Humicola insolens in Pichia pastoris, achieving an enzyme activity yield of 2.36 ± 0.21 U/mL, with optimal performance at pH 7.5 and 70 °C.
- * Using FAE as a pretreatment before chemical bleaching reduced the need for chemical agents by 20%, decreased the kappa value by 10.64%, and improved pulp strength by increasing viscosity by
Studies on the Enzymatic Synthesis and Antioxidant Properties of Phenolic Acid Glycerols.
J Oleo Sci
November 2024
College of Food and Bioengineering, Zhengzhou University of Light Industry.
The potentially wide application of Phenolic acids (PAs) in industries was severely limited by their inadequate solubility and stability in polar/non-polar media. To overcome these limits, studies on the enzymatic esterification of PAs with glycerol were carried out to reach a yield of 95% of phenolic acid glycerols (PAGs) under the following reaction conditions: 1:150 molar ratio of PAs to glycerol; 25% of Lipozyme 435 relative to the weight of total substrates; 80°C, 500 rpm, 86.7 kPa and 10 h.
View Article and Find Full Text PDFDistinct Adjacent Substrate Binding Pocket Regulates the Activity of a Decameric Feruloyl Esterase from .
J Agric Food Chem
October 2024
Laboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Article Synopsis
- The study focuses on how the human gut microbiota processes dietary carbohydrates decorated with ferulic acid (FA), which offers health benefits.
- Researchers presented the crystal structure of a decameric feruloyl esterase (Fae) in complex with methyl ferulate (MFA), illustrating how MFA binds in a noncatalytic pocket that influences enzyme activity.
- Additionally, experiments using molecular docking and quantum mechanics revealed that this adjacent pocket impacts substrate binding and negatively regulates Fae's activity, potentially affecting the metabolism of FA-conjugated polysaccharides in the gut.
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