Preeclampsia is a disorder of pregnancy characterized by endothelial activation. It is believed to be a response to a 'toxin(s)' from the placenta including trophoblastic debris and inflammatory cytokines. Calcium is known to reduce the risk of preeclampsia but the mechanism of its protective effect remains unknown. In this study, we investigated the potential mechanism(s) of calcium supplementation for preventing endothelial activation induced by trophoblastic debris. Trophoblastic debris was harvested from preeclamptic placentae and also from first-trimester placentae, which had been treated with preeclamptic sera. Endothelial cells were then cultured with trophoblastic debris in the presence of calcium. Endothelial activation was measured by quantifying endothelial cell-surface intercellular adhesion molecule-1 (ICAM-1) and by U937 monocyte adhesion to endothelial cells. The expression of ICAM-1 and U937 adhesion to endothelial cells were significantly reduced following exposure of endothelial cells to trophoblastic debris from preeclamptic placenta or from first-trimester placentae treated with preeclamptic sera in the presence of calcium compared with treatment without calcium. The expression of ICAM-1 was also significantly reduced following exposure of endothelial cells to trophoblastic debris with the nitric oxide donor or following treatment of endothelial cells with interleukin (IL)-1β in the presence of calcium. Our study demonstrated that calcium supplementation prevented endothelial cell activation induced by trophoblastic debris from preeclamptic placentae. The nitric oxide synthase (NOS) pathway and anti-inflammatory effects are involved in the action of calcium on endothelial cell activation. These findings may suggest, at least in part, the protective mechanism of calcium supplementation on preeclampsia.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/jhh.2015.73 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Epithelioid trophoblastic tumor in male patients with germ cell tumor: A clinicopathologic analysis of five cases.
Hum Pathol
November 2024
Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA. Electronic address:
Article Synopsis
- Epithelioid trophoblastic tumor (ETT) is a rare type of tumor found in the testis, with only seven cases documented before this study, which adds five more cases from a single institution, making it the largest series reported.
- The patients had a mean age of 44, and most had prior testicular germ cell tumors treated with chemotherapy; ETT appeared in metastatic sites many years later, and its diagnosis was often associated with unusual histological features that can resemble other types of cancer.
- Despite treatment, the outcomes were poor, with two patients dying within 17 months of diagnosis and three others remaining alive with metastatic disease averaging 20 months post-diagnosis, highlighting the aggressive nature of ETT.
Inhibitory Impact of Prenatal Exposure to Nano-Polystyrene Particles on the MAP2K6/p38 MAPK Axis Inducing Embryonic Developmental Abnormalities in Mice.
Toxics
May 2024
Key Laboratory of Human Genetics and Environmental Medicine, School of Public Health, Xuzhou Medical University, Xuzhou 221004, China.
Nanoplastics, created by the fragmentation of larger plastic debris, are a serious pollutant posing substantial environmental and health risks. Here, we developed a polystyrene nanoparticle (PS-NP) exposure model during mice pregnancy to explore their effects on embryonic development. We found that exposure to 30 nm PS-NPs during pregnancy resulted in reduced mice placental weight and abnormal embryonic development.
View Article and Find Full Text PDFHistopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with biovar 2.
J Vet Diagn Invest
May 2023
VISAVET Health Surveillance Centre, Faculty of Veterinary Medicine, Complutense University of Madrid, Madrid, Spain.
Porcine brucellosis, which is caused by biovar (bv) 2, is a re-emerging disease that causes reproductive problems in pigs in Europe. The pathogenesis and lesions of intrauterine infection are poorly characterized; characterization could facilitate the diagnosis and investigation of porcine brucellosis. We collected samples of placentas and fetuses for histologic and microbiologic studies during an outbreak of abortions on a pig-breeding farm in Spain.
View Article and Find Full Text PDFPlacental Findings in Preterm and Term Preeclampsia: An Integrative Review of the Literature.
Rev Bras Ginecol Obstet
July 2021
Department of Obstetrics and Gynecology, Universidade Estadual de Campinas, Campinas, SP, Brazil.
Introduction: Preeclampsia (PE) is a pregnancy complication associated with increased maternal and perinatal morbidity and mortality. The disease presents with recent onset hypertension (after 20 weeks of gestation) and proteinuria, and can progress to multiple organ dysfunction, with worse outcomes among early onset preeclampsia (EOP) cases (< 34 weeks). The placenta is considered the root cause of PE; it represents the interface between the mother and the fetus, and acts as a macromembrane between the two circulations, due to its villous and vascular structures.
View Article and Find Full Text PDFMacrophages and T Lymphocytes in the Ovine Placenta After Experimental Infection With .
Vet Pathol
July 2020
Instituto de Ganadería de Montaña (CSIC-Universidad de León), Grulleros, León, Spain.
Article Synopsis
- This study investigates the immune response in sheep's placenta after infection with toxoplasmosis, focusing on early abortions.
- It was found that parasitic activity in the placenta only appeared 4 weeks after the infection, with distinct immune cell changes observed at different stages of abortion.
- The research suggests that the immune responses from both the maternal and fetal sides of the placenta play a role in the disease's development and vary between early and late abortion cases.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!